柱前衍生高效液相色谱法测定鱼罐头中的组胺

色谱 , 2010, 28(11): 1099-1102. doi: 10.3724/SP.J.1123.2010.01099

柱前衍生高效液相色谱法测定鱼罐头中的组胺

基金项目: 中央级公益性科研院所基本科研业务费项目(2009M02)

Determination of histamine in canned fish by high performance liquid chromatography with pre-column derivatization

建立了一种测定鱼罐头中组胺含量的柱前衍生高效液相色谱(HPLC)方法.样品匀浆后采用高氯酸水溶液超声提取,提取液经丹酰氯衍生后,采用HPLC分离,紫外检测器检测,外标法定量.采用粒径为1.8 μm固定相填料的C18色谱柱,在0.3 mL/min 的流速下,样品的分析时间小于5 min,并可有效地减少流动相消耗,节约成本.组胺在0.08～8.00 mg/L 内线性关系良好,相关系数为 0.999.98;酱煮鲐鱼罐头中组胺在不同浓度水平的平均加标回收率均大于96%,相对标准偏差(RSD)小于2.5%;鱼罐头中组胺的定量限可达5.00 mg/kg.所建立的HPLC方法快速、灵敏度高、重复性好,前处理方法简单,可用于鱼罐头中组胺的测定.

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材料期刊网

柱前衍生高效液相色谱法测定鱼罐头中的组胺

Determination of histamine in canned fish by high performance liquid chromatography with pre-column derivatization

参考文献