建立了浓缩苹果汁中链格孢霉素、链格孢酚、腾毒素、链格孢酚甲醚4种毒素残留量的固相萃取-超高效液相色谱-串联质谱检测方法.样品用水稀释后,用PS DVB固相萃取柱净化,外标法定量.测定时用BEH C18色谱柱(50 mm×2.1 mm, 1.7μm)分离,乙腈和水梯度洗脱,质谱测定采用多反应监测(MRM)模式.4种链格孢霉毒素的测定低限在1.0～5.0μg/L 范围内,加标回收率为77.8% ～117.2% ,相对标准偏差均低于9.7% .该方法灵敏、稳定、可靠,可用于浓缩苹果汁样品中4种链格孢霉毒素的检测和确证.
参考文献
| [1] | Gur N,Karamanoglu S,Gur S.World Appl Sci J,2008,3(5):781 |
| [2] | Tiemanna U,Tomeka W,Schneidera F,et al.Toxicology Letters,2009,186(2):139 |
| [3] | Yang X.Foreign Medical Sciences:Section Hygiene (杨欣.国外医学:卫生学分册),2000,27(3):182 |
| [4] | Fàbrega A,Agut M,Calvo M A.J Food Sci,2002,67(2):802 |
| [5] | da Motta S,Soares L M V.Brazilian J Microbiol,2000,31:315 |
| [6] | Delgado T,Gómez-Cordovés C,Scott P M.J Chromatogr A,1996,731:109 |
| [7] | Fente C A,Jaimez J,Vázquez B I,et al.Analyst,1998,123:2277 |
| [8] | Lau B P Y,Scott P M,Lewis D A,et al.J Chromatogr A,2003,998:119 |
| [9] | Magnani R F,De Souza G D,Rodrigues-Filho E.J Agri Food Chem,2007,55(13):4980 |
| [10] | Sulyok M,Krska R,Schuhmacher R.Anal Bioanal Chem,2007,389(5):1505 |
| [11] | Monbaliu S,Van Poucke C,Van Peteghem C,et al.Rapid Commun Mass Spectrom,2009,23:3 |
| [12] | Scott P M,Weber D,Kanhere S R.J Chromatogr A,1997,765:255 |
| [13] | Andersen B,Smedsgaard J,Jrring I,et al.Int J Food Microbiol,2006,111:105 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%