橄榄醇分子印迹聚合物的制备及评价

色谱 , 2013, 31(6): 587-595. doi: 10.3724/SP.J.1123.2012.12014

橄榄醇分子印迹聚合物的制备及评价

靳亚峰 ^1, , 陈娜 ^2, , 刘润强 ^3, , 陈军 ^4, , 柏连阳 ^5, , 张裕平 ^6,

1.河南科技学院应用化学所,河南新乡,453003

2.河南科技学院应用化学所,河南新乡,453003

3.河南科技学院应用化学所,河南新乡453003;湖南农业大学农药研究所,湖南长沙410128

4.河南科技学院应用化学所,河南新乡453003;湖南农业大学农药研究所,湖南长沙410128

5.湖南农业大学农药研究所,湖南长沙,410128

6.河南科技学院应用化学所,河南新乡,453003

基金项目: Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry and the National Natural Science Foundation of China (30771436)

关键词：分子印迹聚合物 , 固相萃取 , 橄榄醇 , 本体聚合

Preparation and evaluation of molecularly imprinted polymer of olivetol for solid phase extraction

Keywords： molecularly imprinted polymer (MIP) , solid phase extraction , olivetol , bulk polymerization

以橄榄醇为模板分子,α-甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,甲苯和十二醇为溶剂,通过本体聚合法制备了橄榄醇分子印迹聚合物.利用平衡结合实验、扫描电镜(SEM)及红外光谱(FTIR)对分子印迹聚合物(MIP)进行了表征,并用该聚合物进行了加标麦麸中橄榄醇的固相萃取(SPE)研究.平衡结合实验表明MIP对模板分子具有更好的识别性.Scatchard分析表明对橄榄醇分子的吸附存在2类不同结合位点,其中高亲和力结合位点和低亲和力结合位点的解离常数分别为0.021和1.002 mmol/L,相应的最大表观结合量分别为18.74和135.9μmol/g.在优化的固相萃取条件下,MIP固相萃取柱对加标麦麸中橄榄醇的回收率达到97.8％～ 98.8％,相对标准偏差为2.8％～4.2％(n=5),线性范围为0.1～100 mg/L,检出限(S/N =3)为0.062mg/L.与非印迹聚合物(NIP)柱及市售聚苯乙烯/二乙烯基苯(PLS)柱相比,MIP柱的选择性更强,回收率更高,纯化效果更好.

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