肽组学样品前处理方法与技术进展

色谱 , 2013, 31(7): 601-612. doi: 10.3724/SP.J.1123.2013.05030

肽组学样品前处理方法与技术进展

魏黎明 ^1, , 陆豪杰 ^2, , 杨芃原 ^3, , 武欣 ^4,

1.复旦大学生物医学研究院&化学系,上海,200032

2.复旦大学生物医学研究院&化学系,上海,200032

3.复旦大学生物医学研究院&化学系,上海,200032

4.复旦大学附属妇产科医院,女性生殖内分泌相关疾病重点实验室,上海200011

基金项目: 国家杰出青年科学基金项目(21025519)

关键词：肽组学 , 低分子量蛋白质 , 多肽 , 样品前处理

Development of sample pretreatment approach and technology for peptidome

Keywords： peptidome , low-molecular weight proteins , peptides , sample pretreatment

肽组学作为蛋白质组学研究领域的一个重要分支,在生物标志物发现、疾病早期诊断、生物制药等领域有着广阔的应用前景.在肽组学研究过程中,样品前处理是首要环节.本文对肽组学样品前处理方法与技术进行了综述,内容涉及超滤技术、有机溶剂沉淀方法、固相萃取技术等.样品中大量高丰度蛋白质的存在是肽组学发展面临的最大挑战,因此迫切需要发展快速、有效、高通量、自动化的样品前处理方法和技术.

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肽组学样品前处理方法与技术进展

Development of sample pretreatment approach and technology for peptidome

参考文献