建立了测定乳粉中低聚果糖的高效阴离子交换色谱-脉冲安培检测（ HPAEC-PAD）方法。样品经50％（ v／v）乙醇水溶液提取，On Guard RP柱除脂，CarboPac PA200阴离子交换色谱柱（250 mm×3 mm）分离。采用水、0.2 mol／L NaOH溶液和0.4 mol／L NaAc溶液三元梯度淋洗，流速为0.4 mL／min，柱温为30℃，进样量为25μL，脉冲安培检测器检测。蔗果三糖、四糖、五糖在0.05~10 mg／L 范围内线性关系良好，相关系数（ r2）>0.9993；定量限分别为0.02、0.005和0.02 mg／L。在0.5、1.0、5.0 mg／L 添加水平下，蔗果三糖、四糖、五糖的平均回收率为86.0％~114.0％。该方法处理简单、结果准确、灵敏度高，适用于乳粉中低聚果糖的测定。
Fructooligosaccharides( FOS)are usually added in milk powder as a kind of prebi-otic. Thus,quantitative analysis of the FOS is very important for the quality control of milk powder. In this study,a simple method for the simultaneous determination of three FOS com-ponents with degrees of polymerization( DP)3-5 in milk powder was developed by high per-formance anion-exchange chromatography coupled with pulsed amperometric detection ( HPAEC-PAD ). The samples were extracted with 50%( v/v ) ethanol aqueous solution and defatted by an On Guard RP pretreatment column. The separation was performed on a Car-boPac PA200 column by gradient elution using deionized water,0. 2 mol/L NaOH solution and 0. 4 mol/L NaAc solution as the mobile phases. The flow rate was 0. 4 mL/min. The column temperature was 30 ℃;and the injection volume was 25 μL. Good linear response was observed in the concentration range of 0. 05-10 mg/L(r2>0. 999 3). The limits of quantification were 0. 02,0. 005 and 0. 02 mg/L for 1-kestose,nystose and fructofuranosyl-nystose,respec-tively. The mean recoveries varied from 86. 0% to 114. 0% at three spiked levels of 0. 5,1. 0 and 5. 0 mg/L. The short-chain fructooligosaccharides from inulooligosaccharides( IOS)were suc-cessfully separated by the developed HPAEC-PAD method. The method is simple,accurate, sensitive,and helpful for the quality control of milk powder.
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