花气味在维持大戟科（Phyllanthaceae）算盘子属（Glochidion）植物和头细蛾（Epicephala）专性传粉互利共生体系中发挥关键作用。本研究采用动态顶空吸附法（ dynamic headspace adsorption）收集算盘子（ Glochidion puberum）花气味，运用气相色谱-质谱（ GC-MS）联用技术分离并鉴定其化学成分，用峰面积归一化法测得各组分的相对含量。结果表明：算盘子花气味中共鉴定出45种挥发物，主要包含单萜和倍半萜类物质；芳樟醇（38.06％）和β-榄香烯（23.84％）相对含量最高，占总相对含量的61.9％，为主要的气味成分，推测这两种物质可能是吸引传粉头细蛾的重要气味成分。本研究为进一步开展触角电生理检测和生物行为试验来筛选吸引传粉头细蛾的活性物质提供了理论依据。
The floral scent plays the important key role in maintaining the obligate pollination mutualism between Glochidion plants and Epicephala moths. In the study,the dynamic head-space adsorption technique was employed to collect the floral scent emitted by Glochidion pu-berum,gas chromatography coupled with mass spectrometry( GC-MS)was used for the detec-tion and identification of volatile chemical components in headspace samples of flowers from G. puberum. The peak area normalization was used to determine the relative contents of each odour component. The results showed that 45 compounds mainly consisting of monoterpenes and sesquiterpenes were isolated from the floral scent produced by G. puberum. Especially, both linalool( 38. 06%)and β-elemene( 23. 84%)were considered as the major scent compo-nents of G. puberum. It was speculated that linalool and β-elemene may be the two potential compounds attracting female Epicephala moths. The study provided the basic data for further electroantennographic detection and bioassays to identify the compounds having the actual physiological activity to female Epicephala moths.
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