材料期刊网

建立了超高效液相色谱-串联质谱法同时测定食品中香兰素、乙基香兰素、麦芽酚和乙基麦芽酚4种香精的方法。样品用水提取，固相萃取小柱净化，目标化合物采用 UPLCTMBEH C18色谱柱（50 mm×2.1 mm，1.7μm）分离，以甲醇和含0.002 mol／L乙酸铵及0.1％（ v／v）甲酸的水溶液为流动相进行梯度洗脱，采用电喷雾离子源电离、正离子多反应监测模式质谱检测。4种香精在5~500μg／L 或10~1000μg／L 质量浓度范围内线性良好，相关系数均在0.9995~0.9998之间；回收率为75.8％~116％，相对标准偏差（ RSD，n=6）为1.58％~4.01％。该方法灵敏、准确，检测范围广，分析速度快，适合食品中香兰素、乙基香兰素、麦芽酚和乙基麦芽酚4种香精的检测。

A method has been developed for the simultaneous determination of vanillin,ethyl vanillin,maltol and ethyl maltol in foods by ultra performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry( UPLC-ESI-MS/MS ). The sample was extracted with ultrapure water,and purified with SPE column. The target compounds were sep-arated on a C18 column with the gradient elution of methanol and water containing 0. 002 mol/L ammonium acetate and 0. 1%( v/v)formic acid. The four components were determined in the modes of electrospray positive ionization( ESI+)and multiple reaction monitoring( MRM). The linear ranges were 10—1 000 μg/L for vanillin and maltol and 5—500 μg/L for ethyl vanillin and ethyl maltol,with the correlation coefficients more than 0. 999. The recoveries of the four com-pounds ranged from 75. 8% to 116%,with the relative standard deviations( RSDs)of 1. 58%—4. 01%. The method showed good extraction efficiency,high sensitivity and good reproducibili-ty. It is suitable for the simultaneous detection of vanillin,ethyl vanillin,maltol and ethyl mal-tol in foods.