建立了甜樱桃果皮中7种花青苷类物质的超高效液相色谱-串联质谱（ UPLC-MS／MS）定性和定量检测方法。样品经0.1％（ v／v）盐酸甲醇提取液提取，AB-8型大孔吸附树脂净化，在电喷雾离子源正离子（ ESI＋）多反应监测（ MRM）模式下对目标物质进行定性和定量分析。结果表明：7种目标化合物的定量限为0.26~1.42μg／kg；在0~100μg／L范围内呈现很好的线性关系，相关系数（ r2）为0.9964~0.9993；方法的加标回收率为97.2％~105.4％，相对标准偏差（ RSD）为1.9％~5.8％。采用该方法对甜樱桃红色品种“美早”和黄色品种“13-33”成熟果实样品进行了分析，发现主要花青苷类物质的成分和含量存在显著性差异。该方法操作简单，灵敏度高，重复性好，花青苷类物质的分析覆盖面广，可用于甜樱桃中花青苷类成分快速鉴定及含量测定。
A method for the determination of seven anthocyanins in the peel of sweet cherry was developed by ultra performance liquid chromatography-tandem mass spectrometry( UPLC-MS/MS). The sample was extracted by methanol containing 0. 1%( v/v)hydrochloric acid,and then purified by AB-8 macroporous resins. The separation was carried out on a Phenomenex Ki-netex column(100 mm×4. 6 mm,2. 6 μm)with mobile phase of 0. 1%(v/v)formic acid aque-ous solution containing 5 mmol/L ammonium formate and methanol. The sample solution was detected by UPLC-MS/MS with ESI under positive ion and multi reaction monitoring( MRM ) modes. The results showed that the limits of quantification( LOQs)for the seven target com-pounds were 0. 26-1. 42 μg/kg. The seven anthocyanin standards showed a good linearity in the range of 0-100 μg/L with the correlation coefficients(r2)of 0. 996 4-0. 999 3. The average recoveries of the seven anthocyanins were 97. 2%-105. 4%,and the relative standard deviations ( RSDs)were 1. 9%-5. 8%. The mature fruit samples of sweet cherry red variety“Tieton”and the yellow variety“13-33”were analyzed by this method. The results showed that the anthocya-nin composition and contents were significantly different between the two varieties. This meth-od can be used for rapid identification and the determination of anthocyanin components in sweet cherry fruits due to its simple operation,high sensitivity,good reproducibility and cover-ing a wide range of anthocyanins.
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