应用石油醚-乙醇（6∶4，v／v）、30 g／L 十二烷基硫酸钠溶液以及含20 g／L 十六烷基三甲基溴化铵的0.5 mol／L硫酸溶液依次处理烟叶样品，制备得到烟叶酸性洗涤纤维素（ ADF）。采用碱性氧化铜氧化降解方法制备得到 ADF降解产物。以 Ultimate XB C18色谱柱为固定相，甲醇和水为流动相，梯度洗脱，在柱温35℃、流速0.8 mL／min、检测波长280 nm和320 nm条件下，对6种烟叶样品的 ADF降解产物进行了液相色谱分离，得到4个特征色谱峰。以这4个特征色谱峰为考察对象，可研究不同烟叶木质素组成的差异及其与烟叶吸味品质的关系。
The acid detergent fiber( ADF)from tobacco leaves was obtained by treating the sample with petroleum ether-ethanol(6∶4,v/v),30 g/L sodium dodecylsulfate and 0.5 mol/L sulphuric acid containing 20 g/L hexadecyl trimethyl ammonium bromide successively. The ADF was degraded by the alkaline CuO oxidation procedure. In this work,six samples of ADF degra-dation products from tobacco leaves were prepared. The samples were analyzed by using gradi-ent liquid chromatography( LC)where an Ultimate XB C18 column was used as stationary phase,with a mixture of methanol and water as mobile phase,at a column temperature of 35℃ and a flow rate of 0.8 mL/min. Dual wavelengths of 280 nm and 320 nm were chosen for the detection. It was found that there were four characteristic peaks for the ADF degradation prod-ucts. By taking these peaks as research object,the optimum time for the degradation was found to be 5 h and the sample solution could be kept stable within 7 days. The established method may provide a new approach for the studies of the differences between lignin composition in different tobacco leaves and the relationship between lignin content and the smoking quality of tobacco leaves.
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