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建立了化妆品中11种二苯酮类紫外线吸收剂的高效液相色谱分析方法。采用四氢呋喃-甲醇-水或二氯甲烷-水体系提取化妆品中二苯酮类紫外线吸收剂，经离心（5000 r／min），上清液过0.22μm 滤膜后，进入高效液相色谱分析。采用 C18色谱柱进行分离，以0.1％（体积分数）甲酸水溶液（含10 mmol／L 乙酸铵）为流动相 A，以0.1％（体积分数）甲酸甲醇为流动相 B，梯度洗脱。方法加标回收率（ n＝7）为93.4％~103.8％，相对标准偏差为0.1％~4.2％，方法的检出限为4.0~30μg／g，方法的定量限为15~100μg／g。采用该方法对42种市售化妆品检测分析发现，有5种二苯酮类紫外线吸收剂被检出，其中防晒隔离液中二苯酮-3和香水中二苯酮-2的检出量分别为2785μg／g和2106μg／g。研究结果表明，所建立的方法具有良好的回收率、重现性和较高的灵敏度，可用于化妆品中多种二苯酮类紫外线吸收剂的分析。

A sample preparation and analytical method with liquid-liquid extraction( LLE)and high performance liquid chromatography( HPLC ) was developed to detect 11 benzophenone ultraviolet-filters in cosmetics. The target compounds were extracted by the mixed solutions of tetrahydrofuran ( THF )/methanol/water or dichloromethane/water at proper ratios. The extracts were centrifuged and filtered to remove matrix compounds,and then analyzed by HPLC. The separation of analytes was carried out on a Diamonsil-C18 column( 150 mm × 4. 6 mm,5 μm)with 0. 1%( v/v)formic acid aqueous solution( containing 10 mmol/L ammonium acetate)as mobile phase A and methanol containing 0. 1%(v/v)formic acid as mobile phase B. The spiked recoveries of the method( n=7)were 93. 4%-103. 8% with the relative standard de-viations of 0. 1%-4. 2%. The limits of detection( LODs)were in the range of 4. 0-30 μg/g and the limits of quantitation( LOQs)ranged from 15 to 100 μg/g. The method was applied to the determination of 42 cosmetic samples randomly purchased from the supermarket in Dalian. Five benzophenone series were always detected,in which the content of benzophenone-3 in sun-screen cream and the content of benzophenone-2 in perfume were very high and reached 2 785μg/g and 2 106 μg/g,respectively. The results showed that the developed method is efficient, reliable and sensitive,which can be applied to the determination of benzophenones in cosmetics.