建立了一种高效液相色谱同时测定尿液中4种肾脏病常用药物依那普利、氨苯蝶啶、呋塞米及缬沙坦含量的方法。色谱分离选用 WondaSil C18-WR（150 mm×4.6 mm，5μm）；以10.0 mmol／L乙酸铵溶液（pH 3.90）和乙腈为流动相进行梯度洗脱，流速为1.0 mL／min，于254 nm波长下检测，18 min内实现了4种药物的分离分析。结果表明依那普利、氨苯蝶啶、呋塞米、缬沙坦分别在0.15~300 mg／L、0.05~100 mg／L、0.75~750 mg／L、0.05~100 mg／L范围内线性良好，检出限依次为1.38×10-2、7.67×10-3、3.69×10-2、1.16×10-2 mg／L，平均加标回收率在89.49％~99.20％之间，相对标准偏差（ RSD，n=3）在4.12％~9.44％之间。结果表明该方法样品处理简便、快速，结果准确可靠，为肾脏病患者尿液中的治疗药物浓度监测提供了一种新方法。
A high performance liquid chromatographic( HPLC)method was proposed for the simultaneous determination of four drugs for kidney disease,enalapril,triamterene,furosemide and valsartan. After proteins being removed by acetone precipitation method,freeze drying and redissolving in mobile phase,the urine samples were analyzed by HPLC. Chromatographic sepa-ration was performed on a WondaSil C18-WR(150 mm×4. 6 mm,5 μm)in gradient elution mode using 10. 0 mmol/L ammonium acetate aqueous solution( pH 3. 90 ) and acetonitrile as mobile phases at a flow rate of 1. 0 mL/min. The detection wavelength was set at 254 nm. Under the optimized conditions,good linearities were obtained in the range of 0. 15-300 mg/L, 0. 05-100 mg/L,0. 75-750 mg/L,0. 05-100 mg/L,and the detection limits were 1. 38 × 10-2, 7. 67×10-3,3. 69×10-2,1. 16×10-2 mg/L for enalapril,triamterene,furosemide and valsartan, respectively. The recoveries were in the range of 89. 49%-99. 20% with the relative standard deviations( RSDs)among 4. 12%-9. 44%. The method is simple,accurate and effective,and the results showed the method is applicable for the analysis of the four drugs for kidney disea-ses in real urine samples.
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