光学吸收检测器是液相色谱仪最常用的检测器之一,检测池是此种检测器的核心部分,是获得光穿过样品信息的重要部件,其设计影响检测器的性能.为了提高检测器的信噪比,减小检测池引起的色谱峰展宽,需要设计更长光程、更小体积的检测池,同时还要保证一定的光通量.受加工技术的限制,增大光程、减小池体积和保证光通量通常很难兼顾,这对优化检测池的设计和加工、提高检测器性能提出了挑战.本文以光学吸收检测器的检测原理为基础,从增大光程、减小体积、利用全反射等角度综述了检测池设计的研究进展,同时对部分检测池的设计进行了详细说明.这些不同的设计思路和结构对于检测池的设计以及光学吸收检测器的研发具有指导意义.
The optical absorption detector is one of the most commonly used detectors for high performance liquid chromatography( HPLC). As a core part of this kind of detector,the designs of flow cells,where light passes through samples for acquiring samples information,will affect the performance of a detector. In order to enhance the signal to noise ratio of detectors and reduce the bands broadening that come from flow cells,it is necessary to design a flow cell with a longer optical path length and a less cell volume while maintaining the luminous flux. However the limitations of the machining capacity make it difficult to increase the optical path length,reduce the cell volume and keep or increase the luminous flux simultaneously. It is a challenge to optimize the designing and machining of flow cells so as to improve the performance of detectors. This review discusses the development of designing flow cells based on the detection principle in some aspects of increasing the optical path length,reducing the cell volume,taking the advantages of total reflection and so on. At the same time,some of the designs are illustrated in detail. These various ideas and structures are significant refer-ences for designing flow cells and developing optical absorption detectors.
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