建立了正相高效液相色谱测定食物中8种维生素E异构体及维生素A的方法。样品中的维生素E异构体和维生素 A经皂化和液液萃取，Waters ACQUITYTM UPLC BEH Amide色谱柱（150 mm×3.0 mm，1.7μm）分离，体积比为90∶10的正己烷与叔丁基甲基醚-四氢呋喃-甲醇（20∶10∶1，v／v／v）为流动相，荧光检测器和紫外检测器串联检测。4种生育酚在5.0~60.0 mg／L（ r2≥0.9999）、维生素 A 和4种三烯生育酚在0.5~6.0 mg／L（ r2≥0.9996）范围内具有良好的线性，各基质中目标物的检出限在20~60μg／kg之间；9个组分在各基质中的加标回收率为79.2％~114.2％，相对标准偏差（ RSD）为1％~12％。该方法简便、灵敏、可靠、环保，可用于食物中8种维生素E异构体以及维生素 A含量的同时测定。
A synchronous detection method was established by normal-phase( NP )-HPLC to separate and determine eight vitamin E isomers and vitamin A in foods. The sample was pre-treated and then separated on a Waters ACQUITY TM UPLC BEH Amide column( 150 mm × 3. 0 mm,1. 7 μm). The mobile phase consisted of hexane( A,90%)and tert-butyl methyl ether-tetrahydrofuran-methanol(20∶10∶1,v/v/v)(B,10%). The analytes were detected by UV and fluorescence detectors,identified and quantified with external standards. The linear ranges of tocopherols were from 5. 0 mg/L to 60. 0 mg/L( r2≥0. 999 9),and those of tocotrienols and vitamin A were from 0. 5 mg/L to 6. 0 mg/L( r2≥0. 999 6),while the LODs of vitamin E iso-mers and vitamin A were from 20 μg/kg to 60 μg/kg. The method is of high precision,stability and repeatability,and the recoveries were from 79. 2% to 114. 2%. This method is simple,accu-rate and suitable for the analysis of vitamin E isomers and vitamin A in foods.
参考文献
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