在甲酸（α相）和氢氧化钠（γ相）缓冲液形成的移动反应界面的基础上，提出了一种衍生移动反应界面模型。模型表明在α相和γ相之间会形成一个新的β相，β相和α相形成衍生移动反应界面，β相和γ相形成移动界面。为了验证该模型的有效性，该文给出了相关理论及数值推导过程。此外，基于毛细管电泳和自制装置进行了相关实验。结果表明，若使用以前的移动反应界面，实验结果与理论计算存在较大误差，而采用该文提出的衍生移动反应界面，实验数据与理论计算结果高度一致。该文提出的衍生移动反应界面理论及模型对于电泳，特别是毛细管电泳中样品的分离与富集具有重要的意义。
A model of derivatized moving reaction boundary( MRB)is formulated from original MRB formed with formic buffer( phase α)and sodium hydroxide( phase γ). The model shows the formation of a new phase ( phase β)with high pH value originated from phase γ,derivatized MRB created with phases α and β,as well as stationary boundary formed between phases β and γ. To demonstrate the validity of this model,the theoreti-cal and numerical procedures are advanced. At the same time,the experimental procedures are also developed relied on a capillary electrophoresis( CE)and a home-made apparatus. There are evident systemic errors and low agreements between the experimental results and theoretical calculation with the original model of MRB de-veloped in our previous papers. However,there are much high agreements between the experiments and theo-retical computation relied on the model of derivatized MRB developed herein. The model of derivatized MRB to-gether with the relative theoretical and experimental procedures holds evident significance for the design of the new separation and compressive techniques of samples used widely in electrophoresis including CE.
参考文献
| [1] | Cao CX;Fan LY;Zhang W.Review on the theory of moving reaction boundary, electromigration reaction methods and applications in isoelectric focusing and sample pre-concentration[J].The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry,20089(9):1139-1157. |
| [2] | Wang, H.-Y.;Li, S.;Tang, Y.-Y.;Dong, J.-Y.;Fan, L.-Y.;Cao, C.-X..Determination of free acidic and alkaline residues of protein via moving reaction boundary titration in microdevice electrophoresis[J].The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry,201312(12):3544-3551. |
| [3] | Tang, Y.-Y.;Wang, H.-Y.;Chen, L.;Li, S.;Guo, C.-G.;Fan, H.-Z.;Cao, C.-X.;Fan, L.-Y..Novel moving reaction boundary-induced stacking and separation of human hemoglobins in slab polyacrylamide gel electrophoresis[J].Analytical and bioanalytical chemistry,201326(26):8587-8595. |
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