单细胞分析可以揭示生命基本单元———细胞物质组成、生理行为的多样性和差异性，是当今生命分析的主流前沿技术。同时，也对分析技术的定量检测极限、分辨率和精密操控能力提出了新挑战。文章着重从单细胞分离、检测、成像等几个主要方面，综述了单细胞技术研究的最新进展，并对分离和检测技术的发展作了展望。
Single cell analysis can reveal the cellular components and physiological behavior diversity. It is on the cutting?edge of bio?analytical chemistry. Challenges on detection limit, resolution, and precise manipulation of cells are really tougher than ever before. This review includes the developments of several main techniques about separation, detection and imaging. At last, the future developments in separation and detection are discussed.
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