采用微乳液相色谱法同时分离7种水溶性维生素(VB1、VB2、VB6、VB12、叶酸、烟酰胺和VC).考察了微乳流动相体系中表面活性剂、油相、助表面活性剂的种类以及流动相的pH值、柱温等对水溶性维生素分离的影响.优化后微乳体系的组成为:十二烷基硫酸钠(SDS)/聚氧乙烯月桂醇醚(Brij35)/正丁醇/乙酸乙酯/水(质量比为2∶60∶66∶8∶864).色谱柱为Agilent TC C18(250 mm×4.6 mm, 5 μm),柱温为30 ℃,检测波长为254 nm,流速为0.5 mL/min.7种水溶性维生素在20 min内达到基线分离.在4~36 mg/L范围内,7种水溶性维生素的质量浓度与峰面积的相关系数均大于0.9991.不同添加水平下,VB1、VB2、VB6、VC和烟酰胺的平均回收率为93.9%~102.9%.该方法可用于食品和药品中的多种水溶性维生素的分离、鉴别及快速测定.
A novel method was developed for the separation of seven water-soluble vitamins (VB1, VB2, VB6, VB12, VC, folic acid and nicotinamide) with microemulsion liquid chromatography.The impacts of following factors on the separation selectivity were investigated: the type of surfactant, the type of oil phase, the type of co-surfactant, the pH of mobile phase and the column temperature.The optimal conditions for the separation of the seven water-soluble vitamins were as follows: Agilent TC C18 (250 mm×4.6 mm, 5 μm) was used at 30 ℃;the microemulsion mobile phase was sodium dodecyl sulfate (SDS)/polyoxyethylene lauryl ether (Brij35)/n-butanol/ethyl acetate/water (2∶60∶66∶80∶864, mass ratio).Baseline separation of the seven water-soluble vitamins was achieved within 20 min with a flow rate of 0.5 mL/min and the detection wavelength of 254 nm.The calibration curve showed a good linearity with the correlation coefficients above 0.9991 in the linear range from 4 to 36 mg/L.The mean recoveries of VB1, VB2, VB6, VC and nicotinamide were 93.9%-102.9% at different spiked levels.The optimized and validated method can be used for simultaneous determination of water-soluble vitamins in food and pharmaceuticals.
参考文献
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%