采用毛细管区带电泳法(CZE)研究牛凝血酶(B-Thr)、凝血酶核酸适配体(Apt29)与丹参中3种活性物质(丹参素钠(SAS)、原儿茶酸(PA)、阿魏酸(FA))间的相互作用.电泳条件为:毛细管长度40 cm,紫外检测波长214 nm,分离电压15 kV,压力进样3.45 kPa,进样时间5 s.分别固定3种活性药物分子的浓度,改变B-Thr或Apt29的浓度,采用Scatchard方程和非特异性结合方程计算结合常数(Kb),表征药物分子和B-Thr或Apt29的相互作用大小.结果表明,PA与B-Thr结合能力最强,Kb为3.39×104L/mol, FA与B-Thr的Kb为1.05×104 L/mol, SAS与B-Thr未表现出结合能力;在与Apt29的相互作用的研究中显示,SAS基本未与Apt29结合,FA与Apt29结合能力强于PA与Apt29的结合能力, FA、PA与Apt29的Kb分别为1.48×104L/mol和1.32×104L/mol.该研究报道了丹参活性分子与B-Thr、Apt29的相互作用结果,可为核酸适配体(蛋白质)与中药分子相互作用的探索提供新方法,适配体与中药分子相互作用亦可为中药分子的靶点发现、靶向转运提供借鉴.
Investigation on interactions between bovine thrombin (B-Thr), aptamer29 (Apt29) and three active components (salvianic acid A sodium (SAS), protocatechuic acid (PA), ferulic acid (FA)) in Danshen was carried out by capillary zone electrophoresis (CZE).CZE conditions were as follows: capillary length of 40 cm, voltage of 15 kV, injection volume of 3.45 kPa, injection time of 5 s.By changing concentration of B-Thr or Apt29 with fixed levels of the three active components, binding constants (Kb) were calculated by Scatchard and non-specific binding equations to show interactions between B-Thr, Apt29 and the three active components.The results showed that PA had the strongest affinity to B-Thr with Kb 3.39×104L/mol.Kb between FA and B-Thr was 1.05×104 L/mol.SAS had no binding with B-Thr.FA showed stronger affinity with Apt29 than PA with Kb of 1.48×104 L/mol and 1.32×104 L/mol, respectively.The present study reported that interactions between the active constituents in Danshen and B-Thr/Apt29.New methods might be supplied for calculation of interactions between Apt (protein) and herbal drug molecules.And it could give insight for target discovery and delivery of herbal drug molecules.
参考文献
| [1] | Tasset DM.;Steiner W.;Kubik MF..OLIGONUCLEOTIDE INHIBITORS OF HUMAN THROMBIN THAT BIND DISTINCT EPITOPES[J].Journal of Molecular Biology,19975(5):688-698. |
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