通过自由基聚合反应合成了具有高比表面积的介孔硅(PPOSS),并对其表面进行修饰和铜离子(Cu2+)的固定,最终得到功能化介孔硅材料(PPOSS-IDA-Cu2+),然后利用PPOSS-IDA-Cu2+中的Cu2+与蛋白质裸露组氨酸的螯合作用,选择性吸附富含裸露组氨酸的蛋白质.对牛血红蛋白、牛血清白蛋白、肌红蛋白和溶菌酶的吸附结果表明,所合成的PPOSS-IDA-Cu2+材料对富含组氨酸的牛血红蛋白有较好的吸附选择性和较大的吸附容量(3 150 mg/g),并且有望与其他材料联合使用,以检测到更多相对丰度较低的蛋白质,丰富人类蛋白质组学信息,为疾病的临床诊断及病理研究提供帮助.
The Cu2+immobilized mesoporous silica particles (PPOSS-IDA-Cu2+) were fabricated.The prepared materials were applied to the highly specific separation of surface-exposed histidine proteins, based on immobilized metal ion affinity chromatography.The adsorption experiments of bovine serum albumin, myoglobin, lysozyme and bovine hemoglobin (BHb) exhibited that PPOSS-IDA-Cu2+ had excellent performance in the separation of protein BHb.The maximum binding capacity of the proposed materials for BHb was 3 150 mg/g.The results also demonstrated that PPOSS-IDA-Cu2+ had potential application in removing highly abundant proteins in proteomic analysis, and can be combined with other techniques to get more information on low abundant proteins.
参考文献
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