该文建立了大孔树脂-高速逆流色谱分离薇甘菊中黄酮类物质的方法.分离条件为:采用大孔树脂AB-8,洗脱液为50%(v/v)乙醇水溶液,高速逆流色谱溶剂体系为正丁醇-乙酸-水(4∶1∶5, v/v).从薇甘菊中分离到4种黄酮类物质:槲皮素-3-O-芸香糖苷(纯度90.2%)、山奈酚-3-O-芸香糖苷(纯度98.55%)、木犀草苷(纯度98.33%)和紫云英苷(纯度99.23%).建立的大孔树脂-高速逆流色谱方法简单、高效,可扩展应用于从其他植物中分离黄酮类物质.
The present study established a method for the separation and purification of four flavonoids quercetin-3-O-rutinoside, kaempferol-3-O-rutinoside, luteoloside and astragalin from Mikania micrantha by using macroporous resin combined with high speed countercurrent chromatography (HSCCC).Macroporous resin was AB-8 and the eluent was 50% (v/v) ethanol.A two-phase solvent system composed of n-butanol/acetic acid/water (4∶1∶5, v/v) was used in HSCCC.Quercetin-3-O-rutinoside, kaempferol-3-O-rutinoside, luteoloside and astragalin with the purity of 90.12%, 98.55%, 98.33% and 99.23%, respectively, were successfully separated from Mikania micrantha by HSCCC using the lower phase of this solvent system as mobile phase in one run.This study pave a simple and efficient method that could be used in the following separation of flavonoids from invasive plants.
参考文献
| [1] | Aguinaldo AM;Padolina WG;Abe F;Yamauchi T.Flavonoids from Mikania cordata[J].Biochemical Systematics and Ecology,20036(6):665-668. |
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