蛋白质的末端不仅是蛋白质合成的起点和终点,更参与并调节蛋白质的各项生理功能.研究蛋白质的末端不仅对于蛋白质的结构和功能注释十分必要,还为揭示蛋白酶的作用机理提供重要信息.该文着重针对近年来蛋白质组末端肽富集策略方面取得的进展进行综述,并对蛋白质末端组学技术在蛋白酶作用底物和位点分析中的应用进行了概述.此外,还对蛋白质末端组学研究的发展前景进行了展望.
Termini of proteins are not only the origin and destination of protein synthesis, but also participating in and mediating a host of physiological functions of proteins.The determination of proteins termini provides valuable information for protein structure and function annotation, and helps the profiling of proteases substrates and cleavage sites.Herein, the progress in the strategies for enrichment of protein terminus is reviewed.In the meantime, the applications of terminome analysis for the identification of protease substrate and cleavage site are briefly introduced.Finally, the future of terminome research is discussed.
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