建立一种简便、快速、灵敏的高效液相色谱-质谱联用测定老鼠血清中苯扎贝特的方法.采用Gemini C18色谱柱；流动相:V(乙腈)∶V(甲醇)∶V(水)=60∶15∶25(0.03％甲酸水溶液)；柱温35℃,流速0.3 mL/min；紫外吸收波长为235 nm；进样量5μL.质谱采用电喷雾电离负离子模式,用于定量分析的离子分别为[M - H] - m/z 359.65→273.70(苯扎贝特)和[M - H] -m/z 212.95→127.08(氯贝酸).对苯扎贝特检测的线性范围为0.073 ～7.884 mg/L,r=0.9995.得到苯扎贝特的平均回收率为94.3％ ～ 103.1％；方法的日内和日间的相对标准偏差(RSD)均小于6％；最低检测限(LOD)与定量限(LOQ)分别为9.0和30 μg/L;为临床上人体血清中苯扎贝特的浓度检测提供了一种重现性好、灵敏度高的分析方法.
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