A multiphase enzyme membrane reactor using aqueous-organicbiphase instead of water phase alone as the reaction medium was employed to investigate the lipase-catalyzed synthesis of bioactive dipeptides. The medium effect on dipeptide yield was first studied. When N-acetyl-L-phenylalanine ethyl ester（APEE） was used as a carboxyl component, the reactivity order of amino acid amides was found to be L-Leu-NH2＞L-Val-NH2＞L-Ala-NH2＞L-Gly-NH2. The didpetide, N-Ac-L-Phe-L-Leu-NH2, could be synthesized in the multiphase enzyme membrane reactor in a high yield and purity due to the simultaneous separation and reaction.
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