用海藻酸钠(SA)将辣根过氧化物酶(HBP)固定到热裂解石墨电极表面,制备了HBP-SA膜修饰电极.研究结果表明,包埋在SA膜中的辣根过氧化物酶可与电极直接传递电子,在磷酸盐缓冲溶液和磷酸盐/乙醇混合溶液中均可得到1对辣根过氧化物酶辅基血红素Fe(Ⅲ)/Fe(Ⅱ)电对的可逆氧化还原峰.其式电势随溶液pH值增加而负移,呈线性关系,这说明辣根过氧化物酶的电子传递过程伴随有质子的转移.考察了HBP-SA膜修饰电极对O2气、H2O2、NO和六氯乙烷的电催化性质.
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