采用混合型强阳离子固相萃取柱(SPE)对血液中的吗啡、可待因、6-单乙酰吗啡3种鸦片类毒品进行提取,提取液吹干后,再用N-甲基-双三氟乙酰胺(MBTFA)衍生化,进行GC/MS-SIM检测. 以乙基吗啡为内标,3种毒品检测的线性相关系数均>0.99, 线性范围为10~1 000 μg/L,相对回收率分别为90%~116%、90%~110%、79%~102%;日内和日间相对标准偏差分别小于10%和16%;检测限分别为1、0.5和3 μg/L. 该方法灵敏度高、重现性好、操作简便,可用于鸦片类毒品滥用者或中毒者血液中的毒品及其代谢物的检测.
Morphine, codeine and 6-acetyl morphine in human blood were detemined via solid-phase extra-ction and gas chromatography/mass spectrometry-selected ion monitoring, with ethyl morphine as internal standard, 3 opiates spiked in blank blood were extracted wtih a solid-phase cartridge. The fluent was evapo-rated to dryness followed by being derivatized with MBTFA, and then it was analyzed directly by GC/MS in selected ion mode. The method was validated in a range of 10~1 000 μg/L with the drug-spiked blood, and the reactor responded linearly over the studied range(R~2>0.99 for the 3 drugs). The relative standard deviations(RSD) for intra and inter-days are less than 10% and 16% respectively;the relative extraction recoveries are 90%~116%, 90%~110% and 79%~102% sequenfyl for 3 drugs above, and LODs were less than 1, 0.5 and 3 μg/L respectively, which promised the perspective application of the method in detecting trace amounts of opium in human blood.
参考文献
[1] | Meng Q C,Cepeda M S,Kramer T,Zou H,Matoka D J.Farrar J Chromatogr B[J],2000,742:115 |
[2] | ZHANG Dao-Ning(张道宁).Chem Bull(化学通报)[J],1998,3:1 |
[3] | Cordero R,Paterson S.Chromatogr B[J],2007,850:423 |
[4] | Rook E J,Hillenbrand M J X,Rosing H,Ree J M van,Beijnen J H.Chromatogr B[J],2005,824:213 |
[5] | Concheiro M,Castro A de,QuinteLa O,Rivadulla M L,Cruz A.Chromatogr B[J],2006,832:81 |
[6] | BoLeda M R,Galceran M T,Ventura F.Chromatogr A[J],2007,1 175:38 |
[7] | SHEN Min(沈敏).Analysis of Drug Abuse in the Body(体内滥用药物分析)[M].Beijing(北京):Law Publishing House(法律出版社),2003:105 |
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