采用荧光光谱和紫外吸收光谱法研究聚乙烯醇(PEG)和四乙烯五胺(TEPA)与淀粉酶相互作用. 结果表明,PEG会增强淀粉酶内源性荧光和酪氨酸残基所处微环境的疏水性;TEPA对淀粉酶内源性荧光的猝灭机制属于动态猝灭,但同时也存在静态猝灭特征,并使色氨酸残基所处微环境的极性增大;在所考察的范围内, PEG与淀粉酶的结合常数在40 ℃达到最高,TEPA对淀粉酶荧光的动态猝灭结合常数在30 ℃以上趋于最大,PEG、TEPA与淀粉酶之间的作用力属于疏水与静电作用相结合.
Polyvinyl alcohol(PEG) and polyethylenamine(PEI) have been widely applied in the techno-logical fields related to biochemistry. In this work, the interactions of amylase with PEG and tetraethylene pentamine(TEPA) were investigatied by fluorescence and UV absorption spectra. The results show both the inner fluorescence of amylase and the hydrophobicity of the microenvironment of tyrosine residues are enhanced in the presence of PEG. The quenching mechanism of inner fluorescence of amylase by TEPA is dynamic, but also exhibits a feature of static quenching. The polarity of microenvironment of tryptophan residues of increases amylase due to interaction with TEPA. In the range of this investigation, the binding constant of interaction of PEG with amylase reaches the highest value at 40 ℃, the value of quenching constant of binding between TEPA and amylase becomes the maximum at 30 ℃. The interaction of amylse with PEG or TEPA involves hydrophobic and electrostatic interactions.
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