采用京尼平交联壳聚糖溶液制备壳聚糖水凝胶,冷冻干燥后制备壳聚糖细胞支架。研究制备工艺对壳聚糖支架性能的影响,结果表明,在中性环境下,交联制备得到的壳聚糖支架微观呈现均匀的多孔结构,孔隙率在90%以上；有较高的吸水率与蛋白吸附率(36.1 mg/g)；在37℃ PBS环境中,体积溶胀率最低(11.0%)。力学测试表明其具有良好的回弹性,压缩至20%时,撤去应力,仍可恢复原始形貌,且压缩模量最高(81 kPa)。MTT 实验与 SEM观察表明,PC12细胞在此支架中增殖较快。
Chitosan scaffolds were fabricated via freeze-drying chitosan gel using genipin as a crosslinking agent. The influence of the preparation technology to the property of scaffold was studied and the results showed scaf-fold fabricated from a neutral pre-gelled chitosan solution displayed highly interconnected and uniformly distrib-uted pores and a porosity over 90%.This scaffold also showed higher water uptake and BSA adsorption.Com-pressive tests demonstrated that this scaffold had significant elasticity and maintained its physical integrity even after compressing them down to 20% of their original height and the elastic modulus reached 81kPa.Adhesion and proliferation of PC1 2 cells on this scaffold showed excellent properties by MTT and SEM observation, which indicated the potential for nerve tissue engineering applications.
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