以静电纺制备的 PAN 纳米纤维为原料,经偕胺肟基改性后,制得偕胺肟基螯合纳米纤维(AO-PAN)；再利用 AOPAN 纳米纤维上的偕胺肟基与Cu2＋、Co2＋形成配位键,将 Cu2＋、Co2＋固定在 AO-PAN 纳米纤维上,形成金属配合纳米纤维；最后利用金属配合纤维上的 Cu2＋、Co2＋与漆酶形成配位反应,将漆酶固定化。通过红外光谱仪(IR)、扫描电子显微镜(SEM)和透射电子显微镜连接的 X 射线能谱仪(EDAX)对不同处理阶段的 PAN 纳米纤维进行了观察分析,并对固定化漆酶的热稳定性、酸碱稳定性、存储稳定性及重复使用性能进行了研究。结果表明,经过配位反应固定的漆酶最适温度为50℃,最适 pH 值为3,存储稳定性比自由态漆酶高出30％~45％,并且表现出具有一定的可重复使用性。
Electrospun PAN nanofiber was modified into a chelating fiber containing amidoxime group amid-oxime,and then Cu2+ or Co2+ was fixed on the AOPAN nanofibers by coordination bonds to forma chelated nanofibers.Finally,laccase was immobilized by the coordination reaction between laccase and Cu2+ or Co2+.In this paper,the various stages of processing of PAN nanofibers were observed and analyzed by IR,SEM and EDAX.At the same time,the thermal stability,pH stability,storage stability and reuse stability of the immo-bilized laccase were researched.The results showed that the optimum temperature immobilized laccase with che-lated nanofibers was 50 ℃ and the optimum pH value was 3.The storage stability of immobilized laccase in-creased 30%-45% comparing with the free laccase.The immobilized laccase also and had a certain reusability .
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