材料期刊网

研究Ti、Ti6Al4V和 Ti6Al7Nb 3种钛金属表面经喷砂酸蚀处理后的表面形貌、亲水性及对成骨细胞生物活性的影响。在 Ti、Ti6Al4V 和 Ti6Al7Nb 3种钛金属表面进行 Al2 O3喷砂和盐酸、硫酸混合物酸蚀的表面改性处理(SLA ),通过扫描电子显微镜(scanning electron microscope,SEM)观察样品的表面形貌,通过接触角测量仪在显微镜下测量接触角的大小；将SD大鼠成骨细胞以1×104 cells/mL 密度接种于Ti、Ti6Al4V 和 Ti6Al7Nb 表面后通过 MTT 活性实验观察成骨细胞在样品表面的增殖,通过SEM观察细胞在样品表面生长的形态,通过碱性磷酸酶(AKP)活性实验,检测成骨细胞的分化能力。Ti、Ti6Al4V和Ti6Al7Nb 在经过喷砂酸蚀处理后,表面均呈现出微米级多孔形貌,3种样品均为亲水性表面；细胞在 SLA处理后的Ti、Ti6Al4V和Ti6Al7Nb 表面增殖良好,细胞伸展显著；其中在 Ti6Al7Nb 表面细胞的增殖、黏附、分化能力最强。大颗粒喷砂酸蚀技术的表面处理方法能够促进Ti、Ti6Al4V和Ti6Al7Nb 的生物活性；经SLA处理的Ti6Al7Nb 比Ti和Ti6Al4V表现出更好的生物学活性,成骨细胞在其表面呈现出更好的增殖、黏附及分化能力。

Objective:the impact on the biological activity of osteoblasts were investigated after Ti,Ti6Al4V and Ti6Al7Nb were treated by sand blasting and acid etched(SLA).The SLA process was sandblasted by Al2 O3 particles and acid etched by a mixture of hydrochloric acid and sulfuric acid.The sample surface morphologies were characterized by SEM,the contact angles of the samples were measured by angle measuring instrument. Osteoblasts of 1 × 104 cells/mL seeded on Ti,Ti6Al4V and Ti6Al7Nb surface were observed by MTT prolifer-ation tests.The morphologies of osteoblasts were by SEM and the ability to differentiate of osteoblasts were ob-served by alkaline phosphatase (AKP)activity experiments.After the process of SLA,the surfaces of the sam-ples were porous of micro meters and hydrophilic surface.Osteoblasts proliferation and adhesion were better af-ter SLA surface modification.Moreover,proliferation,adhesion and differentiation were best on the surface of Ti6Al7Nb than on the surface of Ti and Ti6Al4V.SLA surface treatment can promote biological activity Ti, Ti6Al4V and Ti6Al7Nb;Ti6Al7Nb treated by SLA showed better biological activity than that of the Ti6Al4V and Ti after SLA,which was significant for implant surface modification.