将儿茶酚(catechol)接枝到聚乙烯醇(PVA)侧基上，得到改性聚乙烯醇(m-PVA)，以模拟贻贝类分泌的黏附蛋白液。通过(UV-spectra)、(FT-IR)及(H 1 NMR)证实了 m-PVA 的大分子结构，并计算出接枝率大约为10％。此外，通过热失重分析(TG)表征了 m-PVA 的热稳定性能。对于模拟贻贝类黏附凝胶的形成机理，通过 UV-spectra 研究了 m-PVA 在不同pH 值条件下对三价铁离子(Fe3＋)的络合作用及所形成的黏附凝胶，以及在高碘酸钠(NaIO 4)的氧化下，通过化学交联得到的具有黏附性质的凝胶。通过本文的初步研究，最终合成出具有一定黏附性质的 m-PVA凝胶，在一定程度上对贻贝类黏附凝胶进行了仿生。
In this work,we grafted polyvinyl alcohol (PVA)with a functionality of adhesive structure,3,4-di-hydroxybenzaldehyde,to obtain a mussel-inspired modified PVA (m-PVA).Firstly,m-PVA was synthesized with PVA and 3,4-dihydroxybenzaldehyde via acetalization.The m-PVA was characterized by UV-spectra,FT-IR and H 1 NMR,which confirmed the catechol was grafted in the polymer.And the grafting ratio is about 10%. In addition,the thermal stability of m-PVA was also measured by TG analysis method.For mimic of mussel ad-hesive protein gel,UV-spectra were used to study the formation of m-PVA adhesive gel,which was obtained by coordinating with Fe3 + with different pH.UV-spectra were also used to investigate the formation of m-PVA ad-hesive gel,which formed by oxidation of NaIO 4 that can oxidize the catechol to form quinone.Through our pri-mary work,adhesive gel was synthesized successively,which mimics mussel adhesive protein gel to some extent.
参考文献
| [1] | Silverman HG;Roberto FF .Understanding marine mussel adhesion[J].Marine biotechnology,2007(6):661-681. |
| [2] | Danner, E.W.;Kan, Y.;Hammer, M.U.;Israelachvili, J.N.;Waite, J.H. .Adhesion of mussel foot protein Mefp-5 to mica: An underwater superglue[J].Biochemistry,2012(33):6511-6518. |
| [3] | Jing Yu;Wei Wei;Eric Danner;Rebekah K Ashley;Jacob N Israelachvili;J Herbert Waite .Mussel protein adhesion depends on interprotein thiol-mediated redox modulation[J].Nature chemical biology,2011(9):588-590. |
| [4] | Stewart, R.J.;Ransom, T.C.;Hlady, V. .Natural underwater adhesives[J].Journal of Polymer Science, Part B. Polymer Physics,2011(11):757-771. |
| [5] | Hu Bi-Huang;Messersmith Phillip B. .Protection of 3,4-dihdroxyphenylalnine (DOPA) for Fmoc solid-phase peptide synthesis[J].Tetrahedron letters: The International Journal for the Rapid Publication of Preliminary Communications in Organic Chemistry,2000(31):5795-5798. |
| [6] | Ronit B;Havazelet B P .Biomimetic adhesives based on al-gae glue[J].Macromolecular Bioscience,2008,8(05):393-400. |
| [7] | Strausberg R L;Link R P .Protein-based medical adhe-sives[J].Trends in Biotechnology,1990,8(02):53-57. |
| [8] | Hwang DS;Sim SB;Cha HJ .Cell adhesion biomaterial based on mussel adhesive protein fused with RGD peptide.[J].Biomaterials,2007(28):4039-4046. |
| [9] | Yamamoto H;Hayakawa T .Conformational studies of sequential polypeptides containing L-β-(3,4-dihydroxy-phenyl)-α-alanine(Dopa)and L-lysine[J].Macromole-cules,1983,16(07):1058-1063. |
| [10] | Holten-Andersen, N.;Harrington, M.J.;Birkedal, H.;Lee, B.P.;Messersmith, P.B.;Lee, K.Y.C.;Waite, J.H. .pH-induced metal-ligand cross-links inspired by mussel yield self-healing polymer networks with near-covalent elastic moduli[J].Proceedings of the National Academy of Sciences of the United States of America,2011(7):2651-2655. |
| [11] | Murat Guvendiren;Phillip B.Messersmith;Kenneth R.Shull .Self-Assembly and Adhesion of DOPA-Modified Methacrylic Triblock Hydrogels[J].Biomacromolecules,2008(1):122-128. |
| [12] | Lee H;Lee Y;Statz AR;Rho J;Park TG;Messersmith PB .Substrate-independent layer-by-layer assembly by using mussel-adhesive-inspired polymers[J].Advanced Materials,2008(9):1619-1623. |
| [13] | Ying Peng;Zhen Zheng;Peiyu Sun .Synthesis and characterization of polyphenol-based polyurethane[J].New Journal of Chemistry,2013(3):729-734. |
| [14] | Westwood G;Horton TN;Wilker JJ .Simplified polymer mimics of cross-linking adhesive proteins[J].Macromolecules,2007(11):3960-3964. |
| [15] | 孙培育,田立颖,郑震,王新灵.含多巴胺的贻贝仿生聚氨酯[J].高分子学报,2009(08):803-808. |
| [16] | 金波,董海山,彭汝芳,沈娟,楚士晋.聚乙烯醇缩间硝基苯甲醛的合成与热稳定性[J].高分子材料科学与工程,2011(06):4-7. |
| [17] | Mary J. Sever;Jaime T. Weisser;Jennifer Monahan;Shalini Srinivasan;Jonathan J. Wilker .Metal-Mediated Cross-Linking in the Generation of a Marine-Mussel Adhesive[J].Angewandte Chemie,2004(4):448-450. |
| [18] | Holowka, EP;Deming, TJ .Synthesis and Cross linking of L-DOPA Containing Polypeptide Vesicles[J].Macromolecular bioscience,2010(5):496-502. |
| [19] | Bum Jin Kim;Dongyeop X Oh;Sangsik Kim .Mussel-Mimetic Protein-Based Adhesive Hydrogel[J].Biomacromolecules,2014(5):1579-1585. |
| [20] | Emilie Faure;Celine Falentin-Daudre;Christine Jerome .Catechols as versatile platforms in polymer chemistry[J].Progress in Polymer Science,2013(1):236-270. |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%