胶原基复合型组织工程化皮肤是未来发展的方向和研究热点。本文在介绍组织工程化皮肤的基础上，简要地综述和探讨了胶原改性的原理与方法、胶原基复合支架材料的造孔方法及其与孔结构之间的关系、种子细胞的选择、细胞与支架材料的复合培养、血管化及其影响因素、及其它皮肤附属器官的再生等关键科学技术问题，并阐明了解决这些科学技术问题的思路和方法，为组织工程化皮肤的研发提供了有益的借鉴。
Collagen-based tissue-engineered skin has become the direction of future development and a hot re-search area.This article briefly reviews the research progress of tissue-engineered skin.Critical science and tech-nology issues of preparation on collagen-based tissue-engineered skin,such as the method of modifying and pore-creating of collagen,the selection of seed cells and its co-culture with the collagen scaffold,the regenera-tion of blood vessel and cutaneous appendages will be also indicated in this article.Additionally,the article pro-vides ideas and methods for solving the science and technology problems and offers beneficial lessons to the study of tissue-engineered skin.
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