利用N-芴甲氧羰基-L-赖氨酸分子接枝异硫氰基荧光素，合成赖氨酸-荧光素小分子，在反相微乳液中京尼平作为交联剂交联赖氨酸-荧光素分子制备超小纳米粒.所得纳米粒纯化后进行紫外-近红外可见光扫描分析，确定荧光素交联到纳米粒上及未反应小分子被完全洗脱.动态光散射考察所得纳米粒径为(198．33±0．03)nm、透射电子显微镜观察到纳米粒呈球形，平均粒径为39．78 nm.荧光光谱表征所得纳米粒与荧光素荧光光谱完全吻合.利用胶质瘤U87细胞进行纳米粒荧光成像，细胞被染色.利用CHO 细胞考察纳米粒的毒性，在一定浓度下(小于8 mg/mL)纳米粒对细胞无明显毒副作用.
Preparation of a kind of ultrasmall fluorescent lysine nanoparticle for Glioblastoma fluorescent ima-ging was as follows:first,the lysine-fluorescein (Lys-FITC)moleculewas synthesizedthrough fluorescein iso-thiocyanateand reaction with amino group of Fmoc-Lys-OH · HCl;second,the end-product Lys-FITC was cross-linked by genipin in reversed microemulsion.After purification,UV absorbance spectrum was investiga-ted to validate no free Fluorescein isothiocyanate remained. Fluorescence property was measured on PerkinElmer LS 5 5 which showed fluorescence spectrum not changed.The particle size and morphology were observed by dynamic light scattering (DLS)and transmission electron microscope (TEM),which showed (198.33±0.03)nm and 39.78 respectively.Finally,Lys-FITC nanoparticle was cultured with U87 cell for fluo-rescent imaging and imaging signal in U87 cell was clearly visible.CHO cell tested by CCK-8 cultured with dif-ferent concentration of nano confirmed no cytotoxicity when bellow 8 mg/mL.
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