材料期刊网

以壳聚糖(Chitosan, CS)和单宁酸(Tannic acid, TA)为组装基元,在水溶液中通过pH诱导组装制备壳聚糖/单宁酸(CS/TA)复合胶体粒子,用透射电子显微镜(TEM)对复合胶体粒子的尺寸及形貌进行了表征.再将复合胶体粒子水分散液为电泳沉积液,通过电泳沉积技术诱导复合胶体粒子在316L不锈钢表面二次组装制备纳米功能涂层;利用接触角测试对涂层表面的亲疏水性进行了研究;并通过体外细胞实验探究了涂层的细胞相容性,抗菌实验测试了涂层对金黄色葡萄球菌和大肠杆菌的抗菌活性.研究结果表明,通过电诱导胶体粒子可以在316L不锈钢表面形成了致密的纳米涂层材料,涂层具有良好的细胞相容性和抗菌作用,在生物涂层领域有着潜在应用前景.

Novel multifunctional coatings were prepared from self-assembled colloidal particles through direct electrophoretic deposition (EPD).The particles were obtained via self-assembly process of chitosan (CS) and tannic acid (TA) in aqueous through hydrogen bonding and electrostatic interactions.The size and morphology of the resulting nanoparticles were successfully studied by transmission electron microscopy (TEM).Multifunctional coating materials were then fabricated in situ on the surfaces of 316L stainless steel by electrophoretic deposition of CS/TA nanoparticles in aqueous.Scanning electron microscopy (SEM) images showed that the particles were uniformly deposited on the surfaces of 316L stainless steel and formed a uniform coating layer.FTIR con?rmed the successful anchoring of CS/TA nanoparticles.Contact angle measurement indicated that the surface hydrophilicity of the substrate was improved by coating materials.More importantly, a fluorescence technique was adopted to qualitative determining the number of L929 cells and bacterial cells adhered on the substrates.The low cytotoxicity of each sample was also evaluated by in vitro test against L929 cells.When directly contacting with bacterial cells, the coatings were able to effectively inactivate both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria.In addition, when compared to bare 316L stainless steel, these coatings effectively promoted L929 cell adhesion and proliferation on the sample surfaces and exhibited excellent antibacterial activity against E.coli and S.aureus in vitro environment.The above results suggested that the coatings could effectively enhance the cytocompatibility and antimicrobial properties of 316L stainless steel substrate.The contribution of this work lies in the creation of a novel strategy to fabricate a multifunctional coating in view of the versatility of self-assembled colloidal particles and the controllability of the EPD process.It is believed that this work could provide new ideas and reliable data to design novel functional coatings.