丝素蛋白/膀胱脱细胞基质/透明质酸复合纤维支架的制备及生物学性能研究

功能材料, 2017, 48(6): 6124-6128. doi: 10.3969/j.issn.1001-9731.2017.06.022

丝素蛋白/膀胱脱细胞基质/透明质酸复合纤维支架的制备及生物学性能研究

蒋丹 ^1, , 黄建文 ^2, , 邵惠丽 ^3, , 胡学超 ^4, , 宋鲁杰 ^5, , 张耀鹏 ^6,

1.东华大学纤维材料改性国家重点实验室,材料科学与工程学院,上海 201620

2.上海交通大学附属第六人民医院泌尿外科,上海,200233

3.东华大学纤维材料改性国家重点实验室,材料科学与工程学院,上海 201620

4.东华大学纤维材料改性国家重点实验室,材料科学与工程学院,上海 201620

5.上海交通大学附属第六人民医院泌尿外科,上海,200233

6.东华大学纤维材料改性国家重点实验室,材料科学与工程学院,上海 201620

基金项目: 国家重点研发计划资助项目(2016YFA0201702/2016YFA0201700) 国家自然科学基金资助项目(21674018,81600524,81671451) 上海市曙光人才计划资助项目(15SG30)

关键词：丝素蛋白 , 膀胱脱细胞基质 , 透明质酸 , 静电纺丝 , 生长因子

Preparation and biological characterization of silk fibroin/bladder acellular matrix /hyaluronic acid electrospun scaffolds

Keywords： silk fibroin , bladder acellular matrix , hyaluronic acid , electrospinning , growth factor

以丝素蛋白(RSF)/膀胱脱细胞基质(BAM)/透明质酸(HA)水溶液为纺丝液,通过静电纺丝法制备RSF/BAM/HA复合纤维支架.通过扫描电子显微镜、ELISA、MTT以及荧光显微镜对支架的形貌和体外生物学性能进行表征.结果表明,3组分比例不变时,纺丝液浓度对纤维支架形貌影响不明显;从支架中成功检测出血管内皮生长因子(VEGF)、血小板衍化生长因子(PDGF-BB)和角化细胞生长因子(KGF)及其缓释行为曲线;这些生物活性因子使RSF/BAM/HA复合纤维支架比纯RSF支架更有利于细胞的粘附与增殖.

Regenerated silk fibroin (RSF)/bladder acellular matrix (BAM)/hyaluronic acid (HA) scaffolds were electrospun from aqueous solution.The morphology and biological performances in vitro were characterized by scanning electron microscope, ELISA, MTT and fluorescent microscopy.Results showed that the concentrations of spinning solutions had limited impact on the morphology of the scaffolds.The existence, bioactivity and continuous release behaviors of vascular endothelial growth factor (VEGF), platelet-derived growth factor BB (PDGF-BB), keratinocyte growth factor (KGF) from the scaffolds were confirmed and determined.Due to the bioactivity of the growth factors, the RSF/BAM/HA scaffolds showed better performance for cell growth and proliferation than bare RSF scaffolds.

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