为了解黄酮类化合物抗氧化构效关系,进一步论证La(Ⅲ)能经代谢调控减轻胁迫伤害.以大豆幼苗为材料,采用水培实验的方法研究了胁迫后期(5d),La(Ⅲ)对UV-B辐射胁迫下大豆幼苗类黄酮含量和类黄酮清除活性氧自由基(O-2;·OH)能力的影响,同时比较了一定浓度下不同类黄酮化合物清除自由基的能力.结果表明,类黄酮含量T1组较CK增加4.82%,T2组较CK降低1.65%;La(Ⅲ)+T1 组较CK增加5.15%,La(Ⅲ)+T2 组较CK降低1.63%,UV-B辐射胁迫对大豆幼苗类黄酮含量有低强度促进高强度抑致的效应; La,UV-B,La(Ⅲ)+UV-B处理组羟基自由基清除率较CK,增幅分别为La(Ⅲ)(3.02%),La(Ⅲ)+T2(2.55%)>T2(-3.68%),La(Ⅲ)+T1(2.36%)> T1(1.13%).La(Ⅲ),UV-B,La(Ⅲ)+UV-B组超氧基清除率较CK,增幅为La(Ⅲ) (3.16%),La(Ⅲ)+T2 (39.02%)>T2(31.40%),La(Ⅲ)+T1(13.95%)>T1(5.58%),La(Ⅲ)可在一定程度上提高大豆幼苗类黄酮含量,增加类黄酮清除自由基能力,减轻UV-B辐射对大豆幼苗伤害.类黄酮清除自由基能力与其组成和构效相关;3种类黄酮清除自由基的能力是槲皮素 >桑色素>芦丁.
参考文献
| [1] | 梁滨,季平扬,唐宋辉,张光生,周青.La对UV-B胁迫下大豆幼苗类黄酮含量影响：Ⅰ对类黄酮总量影响[J].农业环境科学学报,2006(03):584-586. |
| [2] | Noriaki K;Mika K .Enhancement of the tolerance to oxidative stress in cucumber(Cucumis sativus L.) seedlings by UV-B irradiation:Possible involvement of phenolic compounds and oxidative enzyme[J].Plant Res,2000,113(03):311-317. |
| [3] | Murali N S;Teramure A H .Effects of ultraviolet-B irradiance on soybean.VI.Influence of phosphorus nutrition on growth and flavonoid content[J].Physiologia Plantarum,1985,63:413-416. |
| [4] | 韩强,林惠芬,朱玲莉.一些天然提取物对超氧自由基和羟基自由基的清除作用[J].日用化学工业,2000(03):14-17. |
| [5] | 郭亚力,李聪,欧灵澄,王文久,张举成,程伟贤,李云川.3种分光光度法对天然抗氧化物质抗自由基性能的分析检测[J].分析试验室,2004(10):43-48. |
| [6] | Morel I;Lescoat G;Cillard P et al.Role of flavonoids and iron chelation in antixiadant action[J].Methods in Enzymology,1994,234:437-443. |
| [7] | Milic B L;Djilas S M;Canadanovic-brunet J M .Antioxida-tive activity of phenolic compounds on the metal-ion breakdown of lipid peroxidation system[J].Food Chemistry,1998,61(04):443-447. |
| [8] | Kakani V et al.Field crop responses to ultraviolet-B radiation[J].Agricultural and Forest Meteorology,2003,120:191-218. |
| [9] | 杨世园,汪志平,缪云根,朱祥瑞.桑叶粗提物清除羟自由基能力的比较[J].蚕业科学,2005(03):351-353. |
| [10] | 刘杰,王伯初,彭亮,张广求.黄酮类抗氧化剂的构-效关系[J].重庆大学学报(自然科学版),2004(02):120-124. |
| [11] | 王龙,孙建设.类黄酮的化学结构及其生物学功能[J].河北农业大学学报,2003(z1):144-147. |
| [12] | 陈琪,王伯初,唐春红,段传人.黄酮类化合物抗氧化性与其构效的关系[J].重庆大学学报(自然科学版),2003(11):48-51,55. |
| [13] | Gershen Zon J .Change in the levels of plant secondarymetabolites under water and nutrient stress[J].Recent Advances in Phytochemistry,1984,18:273-320. |
| [14] | 邹凤莲,寿森炎,叶纨芝,卢钢.类黄酮化合物在植物胁迫反应中作用的研究进展[J].细胞生物学杂志,2004(01):39-44. |
| [15] | Bors W;Heller W;M Iichel C K.Electron paramagnetic resonance studies of flavonoid compounds[A].Basel:Birkharse,1993:528-530. |
| [16] | Chalxe S L .Environmental significance of anthocyanins inplant stress responses[J].Photochemistry and Photobiology,1999,70:1-9. |
| [17] | Tegelberg R;Julkunen-Tiitto R;Aphalo PJ .The effects of long-term elevated UV-B on the growth and phenolics offield-grown silver birch (Betula pendula)[J].Global change biology,2001(7):839-848. |
| [18] | Markham K R;Ryan K G;Bloor S J et al.A change in the luteolin:apigenin ratio in Marchantia polymorhpa on UV-B enhancement[J].Phytochemistry,1997,48:791-794. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%