将种子液培养与发酵培养结合为一个步骤,并对这种新的低聚果糖合成方法做了初步的基础性研究.研究结果表明,将种子液培养与发酵培养相结合为一个环节,通过优化生产条件,可以使低聚果糖的产率大幅度提高.该法的最适条件为:接种量5%,温度30℃,初始pH7.0,培养时间48 h,初始蔗糖浓度50%,制得的低聚果糖产率为75.21%.该法不仅使寡果糖产率得到大幅度提高,而且可简化工艺,节约资源.
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