本文主要研究基于磁性的CTC捕获芯片中细胞受力情况,提出该类芯片中细胞捕获可分为偏移阶段和吸附阶段,并使用COMOSL软件分析了各个阶段的受力特点.通过模拟计算及实验结果可以看出,传统的垂直捕获模式由于其受流场作用力影响,不适合在高流速下进行CTC细胞捕获.基于此我们提出一种新型的平行捕获模式芯片的设计,该设计可以显著减少流场对CTC细胞捕获的影响.优化后的芯片流速可以达到6ml/h.
参考文献
| [1] | den Toonder J .Circulating tumor cells:the Grand Challenge[J].Lab on A Chip,2011,11(03):375-377. |
| [2] | Sunitha Nagrath;Lecia V. Sequist;Shyamala Maheswaran;Daphne W. Bell;Daniel Irimia;Lindsey Ulkus;Matthew R. Smith;Eunice L. Kwak;Subba Digumarthy;Alona Muzikansky .Isolation of rare circulating tumour cells in cancer patients by microchip technology[J].Nature,2007(7173):1235-1239. |
| [3] | Stott, S.L.;Hsu, C.-H.;Tsukrov, D.I.;Yu, M.;Miyamoto, D.T.;Waltman, B.A.;Michael Rothenberg, S.;Shah, A.M.;Smas, M.E.;Korir, G.K.;Floyd Jr., F.P.;Gilman, A.J.;Lord, J.B.;Winokur, D.;Springer, S.;Irimia, D.;Nagrath, S.;Sequist, L.V.;Lee, R.J.;Isselbacher, K.J.;Maheswaran, S.;Haber, D.A.;Toner, M. .Isolation of circulating tumor cells using a microvortex-generating herringbone-chip[J].Proceedings of the National Academy of Sciences of the United States of America,2010(43):18392-18397. |
| [4] | Ye Xu;Joseph A. Phillips;Jilin Yan;Qingge Li;Z. Hugh Fan;Weihong Tan .Aptamer-Based Microfluidic Device for Enrichment, Sorting, and Detection of Multiple Cancer Cells[J].Analytical chemistry,2009(17):7436-7442. |
| [5] | Joo H. Kang;Silva Krause;Heather Tobin .A combined micromagnetic-microfluidic device for rapid capture and culture of rare circulating tumor cells[J].Lab on a chip,2012(12):2175-2181. |
| [6] | Kazunori Hoshino;Yu-Yen Huang;Nancy Lane .Microchip-based immunomagnetic detection of circulating tumor cells[J].Lab on a chip,2011(20):3449-3457. |
| [7] | J. Xia;X. Chen;C.Z. Zhou;Y.G. Li;Z.H. Peng .Development of a low-cost magnetic microfluidic chip for circulating tumour cell capture[J].IET nanobiotechnology,2011(4):114-120. |
| [8] | 王聿佶,陈翔,潘欣欣,金庆辉,赵建龙.集成高梯度磁场分离结构的微流控芯片快速制作法[J].功能材料与器件学报,2008(04):751-756. |
| [9] | 潘欣欣,陈翔,陈迪.微流控生物芯片的磁场仿真及实验对比[J].微计算机信息,2009(19):189-191. |
| [10] | 潘欣欣,陈翔,陈迪,朱军,高峰.新型纳米磁色谱微流体芯片[J].功能材料与器件学报,2008(02):441-443. |
| [11] | 胡锐军,陈迪,陈翔,陈景东,吴澄,杜航向,杨治力,王志刚,夏骏.一种MEMS结肠癌细胞捕获芯片研究[J].功能材料与器件学报,2011(02):227-232. |
| [12] | Kazunori Hoshino;Peng Chen;Yu-Yen Huang;Xiaojing Zhang .Computational Analysis of Microfluidic Immunomagnetic Rare Cell Separation from a Particulate Blood Flow[J].Analytical chemistry,2012(10):4292-4299. |
| [13] | Kazunori Hoshino;Yu-Yen Huang;Nancy Lane .Microchip-based immunomagnetic detection of circulating tumor cells[J].Lab on a chip,2011(20):3449-3457. |
| [14] | Truskey G A;Proulx T L.Relationship between 3T3 cell spreading and the strength of adhesion on glass and silane surfaces[M].,1993 |
| [15] | Yuan Wan;Jifa Tan;Waseem Asghar .Velocity Effect on Aptamer-Based Circulating Tumor Cell Isolation in Microfluidic Devices[J].The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical,2011(47):13891-13896. |
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