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利用中能重离子束辐照生物靶材料时产生的中子、γ射线和次级碎片等次级辐射对春麦种子进行辐照,然后通过田间试验和实验室的分析测定,得到了M1代植株的变化. (1)在生物学性状中,穗粒数、小穗数、千粒重、穗粒重、小穗密度和有效分蘖数变异率较大; (2)除SOD活性外, POD活性、 CAT活性、 MDA含量和蛋白质含量的变异率也较大; (3)休眠种子和萌发种子辐照M1代在生物学性状的变化上存在很大的差异; (4)两个春麦材料的辐射敏感性差异明显. 由此表明,兰州重离子加速器辐照终端在进行生物学实验时产生的次级辐射是可能利用的诱变源.

Secondary radiation such as neutrons, high energy light particees, γ rays is produced when ion beams with medium energy irradiate biological materials. Spring wheat was placed in radiobiological experiment area(irradiation terminal L2) to expose to the secondary radiation. The variation of M1 generation of the wheat seeds was obtained through test in the fields and measuremeat in laboratery: (1) There were very high variation rates in grain number, number of small ear, 1 000 grain weight, ear grain weight, density of small ear and effective tilling number. (2) The variation rates of POD activity, CAT activity, MDA content and protein content were great, except for SOD activity. (3) There were significant differences of M1 generation biological traits between resting seeds and sprout seed. (4) Radiation sensitivity of two kinds of spring wheat presented obvious difference.   The results indicate that, the secondary radiation is a usable resource in radiobiological research on HIRFL when experiment are in progress.

参考文献

[1] 唐靖宇. 对HIRFL几个主要参数的稳定性要求[J]. 原子核物理评论, 1998, 15(1): 31-34.
[2] 党秉荣, 卫增泉, 王经, 等. 75 MeV/u12C离子束在辐射生物区产生的中子角分布[J]. 辐射防护, 1999, 19(2): 140-143.
[3] 党秉荣, 卫增泉, 王经, 等. 75 MeV/u16O离子在辐射生物试验区产生的中子角分布[J]. 核技术, 1998, 21(9): 525-527.
[4] 颜一鸣. 原子核物理学[M]. 北京: 原子能出版社, 初版, 1990.
[5] 罗景桂. 放射生物学[M]. 北京: 中国农业出版社, 第一版, 1996, 144-171.
[6] Heath R, Packer L L. Photoperoxidaton in Isolated Chloroplasts. I Kinetics and Stoichiometry of Fatty Acid Peroxidation[J]. Arch Biochem Biophys, 1968, 12(5): 189-198.
[7] Del Longo O T, Gonzalez C A, Postori G M, et al. Antioxidant Denfenses under Hyperoxygenic and Hyperosmotic Conditions in Leaves of Two Lines of Maize with Differential Sensitivity to Drought[J]. Plant Cell Physiol, 1993, 34: 1 023-1 028.
[8] Kar P K, Choudhuri M A. Possible Mechanisms of Light-induced Chlorophyll Degradation in Senescing Leaves of Hydrila Verticillata[J]. Physiol Plant, 1987, 70: 729-734.
[9] Aebi H. Catalase in Vitro[J]. Meth Enzymol, 1984, 105: 121-126.
[10] Bradford M M. A Rapid and Sensitive Method for Quantiation of Microgram Quantities of Protein Utilizing the Principle of Protein Dye Binding[J]. Anal Biochem, 1976, 72: 248-254.
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