随着多通道探测技术、集成显微技术和计算机及分析技术的发展,红外和拉曼光谱技术及光谱显微成像技术在生物及医学领域得到广泛应用并成为一种向实用化发展的趋势.但是,要从光谱测量中获得准确、有用的信息需要对光谱及成像数据进行细致、合理的分析.应用MATLAB软件编程,结合单谱线分析和多谱线多变量分析的方法,对健康人和病人头发横截面微观扫描的拉曼光谱数据进行分析,结果表明,通过对头发拉曼光谱细致处理和数据分析,可以揭示健康人和癌症患者之间的差异.这项工作是把拉曼光谱成像技术及相关光谱分析方法应用于生物医学领域的一个实例.
参考文献
| [1] | Bhargava R,Levin I W.Spectrochemical Analysis Using Infrared Multichannel Detectors[M].Oxford England:Blackwell Publishing Ltd.,2005. |
| [2] | Huang Qing.Application of optical spectroscopy in biological research[J].Journal of Anhui University(安徽大学学报(自然科学版)),2012,36(1):6-17 (in Chinese). |
| [3] | Li Yongzeng,Xie Shusen,Chen Rong.Application and technique advances of Raman spectroscopy in human tissues[J].Chin.J.Laser Med.Surg.(中国激光医学杂志),2011,20(3):177-185 (in Chinese). |
| [4] | Liu Jinghua,Huang Qing.Application of FT-IR spectroscopy in study of biological effects on microorganisms induced by ionizing radiation[J].Acta Biophysica Sinica(生物物理学报),2012,28(9):719-730 (in Chinese). |
| [5] | Liu Jinghua,Qi Zeming,Huang Qing,et al.Study of energetic-particle-irradiation induced biological effect on rhizopus oryzae through synchrotron-FTIR micro-spectroscopy[J].J.Mol.Struct.,2013,1031:1-8. |
| [6] | Bhargava R.Towards a practical Fourier transform infrared chemical imaging protocol for cancer histopathology[J].Analytical and Bioanalytical Chemistry,2007,389(4):830-845. |
| [7] | Shafer-Peltier K,Haka A,Motz J,et al.Model-based biological Raman spectral imaging[J].Journal of Cellular Biochemistry,2002,39:125-137. |
| [8] | Krafft C,Steiner G,Beleites C,et al.Disease recognition by infrared and Raman spectroscopy[J].Journal of Biophotonics,2008,2:13-28. |
| [9] | Krafft C,Dietzek B,Popp J.Raman and CARS spectroscopy of cells and tissues[J].Analyst,2009,134:1046-1052. |
| [10] | Lasch P,Haensch W,Naumann D,et al.Imaging of colorectal adenocarcinoma using FT-IR microspectroscopy and duster analysis[J].Biochim.Biophys.Acta,2004,1688:176-186. |
| [11] | Schlücker S,Liang C,Strehle K R.Conformational differences in protein disulfide linkages between normal hair and hair from subjects with trichothiodystrophy:A quantitative analysis by Raman microspectroscopy[J].Biopolymers,2006,82:615-622. |
| [12] | Kuzuhara A,Fujiwara N,Hori T.Analysis of intemal structure changes in black human hair keratin fibers with aging using Raman spectroscopy[J].Biopolymers,2007,87:134-140. |
| [13] | Eilers P H C.A perfect smoother[J].Anal.Chem.,2003,75:3631-3636. |
| [14] | Venkatachalam V,Aravena J L.Nonstationary signal classification using pseudo power signatures:The matrix SVD approach[J].IEEE Transactions on Automatic Control,1999,46(12):1497-1505. |
| [15] | Lattin J M,Green P E,Jr.Carroll D.Analyzing Multivariate Data[M].Beijing:China Machine Press,2003:83-310. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%