研制了一套基于Linnik干涉仪的全场光学相干层析(FF-OCT)系统对细胞进行层析成像.不同于其他FF-OCT系统的成像方法,该系统采用了基于Hilbert变换的图像恢复算法,只需2幅移相干涉图就可以恢复样品的结构信息,即使移相出现误差,仍能得到样品层析图.系统的实测纵向、横向分辨率分别达到1.1 μm、2.5 μm.通过对洋葱表皮细胞的初步成像实验,验证了该FF-OCT系统的可行性,为进一步实现高分辨率细胞成像奠定了基础.
A Linnik type full-field optical coherence tomography (FF-OCT) system is developed for tomographic imaging of biological cells.Different from other imaging methods used in FF-OCT systems,an image restoration method based on Hilbert transform is adopted,which requires only two interference images to recover the structure information of the sample.Even if the phase shift error occurs,the sample chromatogram can still be obtained.The measured longitudinal and lateral resolutions of the system reach 1.1 μm and 2.5 μm,respectively.Feasibility of the FF-OCT system is verified by preliminary imaging experiments of onion epidermal cells,and it lays foundation for the further realization of high resolution cell imaging.
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