研究了膜式糖化反应器及酶回收的工艺过程.首次提出了PS-Ti复合管式膜糖化器水解蔗渣喷爆物及循环使用纤维素酶的新工艺.研究了水解酶失活的机理为非降解底物的吸附作用,即水解酶大部分失活是由于吸附于非降解的蔗渣上所致.超滤与丹宁沉淀法结合,使水解酶回收率均大于90%.膜式糖化反应器的半连续操作的最高总转化率可达97%,在20 h内总转化率水平比间歇提高7倍,还原糖产率提高6倍.水解液中葡萄糖与木糖的质量比为3:1.
参考文献
| [1] | Indisch M R .Cellulose structure modification and hydrolysis[J].Process Biochemistry,1979,14:21-28. |
| [2] | BROWNING B L.The chemistry of wood[M].Interscience New York,1963:429-446. |
| [3] | Wright J D .Ethanol from lignocellulose: an process[J].Process Biochemistry,1995,8(02):71-77. |
| [4] | Ha hn- Higerdal B .Enzymatic hydrolysis of sodium- hydroxide- pretreated sallow in an ultrafiltration memebrane reactor[J].Biotechnology and Bioengineering,1984,26:447-456. |
| [5] | 索里拉金 .反渗透科学[J].膜科学与技术,1984,4(01):44-55. |
| [6] | 宋桂经;高培基;王祖农.纤维素酶的回收[J].生物工程学报,1988(04):65-69. |
| [7] | 杨斌 .甘蔗渣转化为酒精的研究[D].华南理工大学,1996. |
| [8] | Belfort G .Membranes and bioreactors:a technical challenge in biotechnology[J].Biotechnology and Bioengineering,1989,33:1047-1059. |
| [9] | Driol E .生物工程中的膜[J].膜分离科学与技术,1986,2(04):35-41. |
| [10] | 亢茂德;薛伟明;张效林 .酶-膜法药用植物有效成分提取过程[J].化学工程,1994,22:54-66. |
| [11] | 亢茂德;张效林;王科忍 .CA-Ti复合管式膜的超滤传质机理[J].化学工程,1985,13(03):42-48. |
| [12] | 杨斌;李宝璋;高孔荣 .CA-Ti复合管式膜在连续乙醇耦合发酵过程中的膜性能研究[J].膜科学与技术,1995,15(04):11-15. |
| [13] | 北京大学生物系生物化学教研室.生物化学实验指导[M].北京:高等教育出版社,1978:22. |
| [14] | FEN L .Kinetics studies of enzymatic hydrolysis of insoluble cellulose: derivation of mechanistic kinetic model[J].Biotechnology and Bioengineering,1983,25:2752-2766. |
| [15] | Lowry O M.Bioconversion of cellulsic substrates into energy chemicals and microbial protein[J].Journal of Biochemistry,1951:193-265. |
| [16] | Ghose T K .Measurement of cellulase activities[J].Pure and Applied Chemistry,1987,59(02):256-267. |
| [17] | 王玉万;徐文玉 .木质纤维素固体基质发酵物中半纤维素、纤维素和木素的定量分析程序[J].微生物学通报,1987,14:81-88. |
| [18] | Howell H A .Dissesertation enzymatic hydrolysis of cellulose[J].Chemical Engineering Research and Design,1984,62:373-379. |
| [19] | Berghem L E;Pettersson L G .Hydrolysis of pretreated lignocellulosle from crop residue[J].Biotechnology and Bioengineering,1975,53:55-63. |
| [20] | Matson S L;Quinn J A.Membrane reactors in bioprocessing[M].Biochemical Engineering I.Th New York Academy of Sciences,1986:152. |
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