为确定适合胶体金诊断试剂盒使用的膜材料,对几种膜材料的性能进行了定性的分析和筛选,其中包括:混合纤维素膜、硝酸纤维素膜、Predator膜、硝化纤维素膜、玻璃纤维素膜.比较了这几种膜的表面结构、层析性能、层析灵敏度、残留背景色和蛋白的吸附量等指标,结果表明,硝酸纤维素膜和Predator膜适合作胶体金试纸的固相支持膜,玻璃纤维素膜适合作胶体金-蛋白结合物的载体膜.同时又比较了同一材质、不同孔径的硝酸纤维素膜的各种性能,结果表明:硝酸纤维素膜2更适用于竖直爬流式的检测试纸.对近似孔径、不同材质的硝酸纤维素膜2和Predator膜2比较结果说明,Predator膜2更适用于水平泳动式的检测试纸.
参考文献
| [1] | Faulk W P;Taylor G M .An immunocolloidal gold method for the electron microscope[J].Immunocytochemistry,1971,8:1081-1087. |
| [2] | Lu S;Wang S;Chang W B .Application of colloidal gold on immunoassay[J].Nat Sci Ed,2000,146:393-399. |
| [3] | Frens G .Controlled nucleation for the regulation of the partical size in monodisperse gold suspensions[J].Nature Physical Science,1973,242:20-22. |
| [4] | Bardorff M M;Freitag H;Scheffold T et al.Development and characterization of rapid assay for bedside determinations of cardiac troponin T[J].Circulation,1995,92(02):869. |
| [5] | Morais S;Maquieira A;Puchades R .Selection and characterization of membranes by means of an immunofiltration assay.Application to the rapid and sensitive determinatin of the insecticide carbaryl[J].Journal of Immunological Methods,1999,224:101-109. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%