膜错流过滤可实现混合液中物质的分级,在生物工程领域有着广泛的应用,其过滤特性与水力条件及溶液性质有密切关系.分别采用0.45 μm、0.20 μm微滤膜和截留分子量3 000、100 000超滤膜,对其错流过滤透明质酸发酵液的特性参数进行了研究.超滤膜在稳态时的通量大于微滤膜稳态时的通量,微滤膜、超滤膜的最佳操作压力分别为0.1 MPa,0.15 MPa,最佳流速应为1.25 m/s,透明质酸浓度以2 g/L以下为宜.在pH=4,增加的离子强度为0.2～0.25 mol/L NaCl时,微滤膜对透明质酸有85%的透过率,且通量增大,而菌体被完全截留;超滤膜在pH=7,增加的离子强度为0.2～0.25 mol/L NaCl时,蛋白质对透明质酸有很高的筛分系数,透明质酸被选择性截留.研究表明,微滤和超滤相结合可实现发酵液中透明质酸分离.
参考文献
| [1] | Kakeh K;Kinoshita M;Yasueda S .Hyaluronic acid:separation and biological implications[J].Journal of Chromatography B:biomedical Sciences and Applications,2003,797:347-355. |
| [2] | Lisa Jarvis .Hyaluronic Acid Stretches Beyond Cosmetics Into Pharmaceutical Applications[J].Chemical Market Reporter,2002(6):10-0. |
| [3] | D. H. Vynios;N. K. Karamanos;C. P. Tsiganos .Advances in analysis of glycosaminoglycans: its application for the assessment of physiological and pathological states of connective tissues[J].Journal of chromatography, B. Analytical technologies in the biomedical and life sciences,2002(1/2):21-38. |
| [4] | Yamamoto O;Morikawa T;Kitwa H .Manufacture of hyaluronic acid by fermentation,involves culturing hyaluronic acid producing microorganism in a culture solution having the hydrogen carbonate ion concentration maintained at a predetermined value[P].JP 2000189185A,2000-09-11. |
| [5] | Stekolnikov L I;Kornilova A A .Production of hyaluronic acid-includes aerobic fermentation of specified microorganism on aqueous culture medium,and separation by centrifuging and chromatographic purification with ion-exchange resin[P].RU 2074863Cl,1997-03-10. |
| [6] | Silhankova L;Demnerova K;Kralova B et al.Hyaluronic acid prepn.by fermentation of mutant strain of Streptococcus zooepidemicus[P].CS 8904067A2,1992-01-15. |
| [7] | Miyamori T;Numazawa R;Sakimae A et al.Method of producing hyaluronic acid[P].US 4885244,1989-12-05. |
| [8] | Nimrod A;Greenman B;Kanner D .Method of producing high molecular weight sodium hyallronate by fermentation of streptococcus[P].US 4780414,1988-10-18. |
| [9] | Westphal G;Hahn M;Lippert E et al.Isolation of hyaluronic acid,e.g.from fermentation solutions,comprises precipitation with an ethoxylated fatty amine hydrochloride,useful in medical,pharmaceutical and cosmetic applications[P].DE 10019868A1,2002-11-02. |
| [10] | Guojun Zhang;Zhongzhou Liu;Liang Zhao;Hongbing Li;Quan Zhou;Fei He;Zhaohui Xu;Huanzhang Wang .Recovery of glutamic acid from ultrafiltration concentrate using diafiltration with isoelectric supernatants[J].Desalination: The International Journal on the Science and Technology of Desalting and Water Purification,2003(1):17-26. |
| [11] | Shiu-Lan Li;Kan-Sen Chou;Jun-Yu Lin;Hong-Wei Yen;I-Ming Chu .Study on the microfiltration of Escherichia coli-containing fermentation broth by a ceramic membrane filter[J].Journal of Membrane Science,1996(2):203-210. |
| [12] | Lo Y M;Yang S T;Min D B .Kinetic and feasibility studies fo ultrafiltration of viscous xanthan gum fermentation broth[J].Journal of Membrane Science,1996,117:237-249. |
| [13] | Bracke J W;Thacker K .Hyaluronic acid from bacterial culture[P].US 4517295,1985-05-14. |
| [14] | Ellwood D C;Evans C G;Dunn G M et al.Production of hyaluronic acid[P].US 5563051,1996-10-08. |
| [15] | Punidadas P;Rizvi SSH .Separation of milk proteins into fractions rich in casein or whey proteins by cross flow filtration[J].Food Research International,1998(4):265-272. |
| [16] | Bitter T;Muir H.A modified uronic acid carbazole reaction[J].Analytical Biochemistry,1962(04):330-333. |
| [17] | Shimada E;Nakamura K T .Determination of Stokes radii and molecular masses of sodium hyaluronates by Sephacryl gel chromatography[J].Journal of Chromatography A,1994,685:172-177. |
| [18] | Chang-Won Cho;Do-Youn Lee;Chan-Wha Kim .Concentration and purification of soluble pectin from mandarin peels using crossflow microfiltration system[J].Carbohydrate Polymers: Scientific and Technological Aspects of Industrially Important Polysaccharides,2003(1):21-26. |
| [19] | Rossignol N;Vandanjon L;Jaouen P;Quemeneur F .Membrane technology for the continuous separation microalgae/culture medium: compared performances of cross-flow microfiltration and ultrafiltration[J].Aquacultural Engineering: An International Journal,1999(3):191-208. |
| [20] | Gura E.;Mullet PJ.;Huckel M. .Specific degradation of hyaluronic acid and its rheological properties[J].Polymer Degradation and Stability,1998(1/3):297-302. |
| [21] | Fouissac E;Milas M;Rinaudo M et al.Influence of the Ionic Strength on the Dimensions of Sodium Hyaluronate[J].Macromolecules,1992,25(21):5613-5617. |
| [22] | Gill W N;Wiley D E;Fell J D et al.Effect of viscosity on concentration plarixation in ultrafiltration[J].AICHE Journal,1988,34:1563-1567. |
| [23] | Wang S S .Effect of solution viscosity on ultrafiltration flux[J].Journal of Membrane Science,1988,39:187-194. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%