用中空纤维微孔疏水膜进行木瓜蛋白酶的渗透膜结晶,研究温度和不同pH的缓冲溶液对木瓜蛋白酶静态渗透膜结晶的影响,优化木瓜蛋白酶渗透膜结晶的结晶条件.通过监控结晶溶液中木瓜蛋白酶浓度随时间的变化,考察沉淀剂和添加剂对结晶过程的影响,并对晶体进行尺寸分布分析.结果表明,对于木瓜蛋白酶的渗透膜结晶,在15～32℃下,以pH 4.7的乙酸盐为缓冲溶液,在沉淀剂(硫酸铵和磷酸钠)质量分数4%时,制备出质量较好的木瓜蛋白酶晶体.添加剂PEG600和PEG4000的加入提高了溶剂跨膜通量,改善了晶体质量.
参考文献
| [1] | 汪家政;范明.蛋白质技术手册[M].北京:科学出版社,2002:265. |
| [2] | Curcio E;Criscuoli A;Drioli E.Membrane crystallization of macromolecular solutions:the albumin study[A].Cetraro:Italy,2001 |
| [3] | Curcio E;Profio G D;Drioli E .Membrane crystallization of macromaolecular solutions[J].Desalination,2002,145:173-177. |
| [4] | Gianluca Di Profio;Efrem Curcio;Alberto Cassetta;Doriano Lamba;Enrico Drioli .Membrane crystallization of lysozyme: kinetic aspects[J].Journal of Crystal Growth,2003(3/4):359-369. |
| [5] | Curcio E;Simone S;Profio G D et al.Membrane crystallization of lymzyme under forced solution flow[J].Journal of Membrane Science,2005,257:134-143. |
| [6] | Efrem Curcio;Gianluca Di Profio;Enrico Drioli .A new membrane-based crystallization technique: tests on lysozyme[J].Journal of Crystal Growth,2003(1/2):166-176. |
| [7] | Di Profio G;Curcio E;Drioli E .Trypsin crystallization by membrane-based techniques[J].Journal of Structural Biology,2005(1):41-49. |
| [8] | Zhang Xinmiao;EL-Bourawi M S;Wei Kegui et al.Precipitants and additives on membrane crystallization of lysozyme[J].Biotechnology Letters,2006,1:1302-1311. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%