DNA甲基化作为一种十分重要的表观遗传修饰途径,在生物体生长发育过程中发挥着极为关键的作用.甲基胞嘧啶、羟甲基胞嘧啶、醛基胞嘧啶和羧基胞嘧啶是整个DNA甲基化和去甲基化过程中重要的中间体形式,它们的分布含量直接影响着DNA甲基化和去甲基化过程的动态平衡,进而影响着整个正常生理活动的进行.检测这些特殊胞嘧啶在表观遗传学上具有重要的意义.本文小结和综述了本课题组通过化学方法在甲基胞嘧啶、羟甲基胞嘧啶、醛基胞嘧啶和羧基胞嘧啶检测方面取得的最新研究进展.
参考文献
| [1] | Klose RJ;Bird AP .Genomic DNA methylation: the mark and its mediators[J].Trends in biochemical sciences,2006(2):89-97. |
| [2] | Smith,Z.D.;Meissner,A. .DNA methylation: Roles in mammalian development[J].Nature reviews. Genetics,2013(3):204-220. |
| [3] | Baylin SB;Jones PA .A decade of exploring the cancer epigenome - biological and translational implications.[J].Nature reviews Cancer,2011(10):726-734. |
| [4] | Wu SC;Zhang Y .Active DNA demethylation: many roads lead to Rome.[J].Nature reviews: molecular cell biology,2010(9):607-620. |
| [5] | Bhutani, N.;Burns, D.M.;Blau, H.M. .DNA demethylation dynamics[J].Cell,2011(6):866-872. |
| [6] | Kriaucionis S;Heintz N .The nuclear DNA base 5-hydroxymethylcytosine is present in purkinje neurons and the brain[J].SCIENCE,2009,324(5929):929-930. |
| [7] | Tahiliani M;Koh K P;Shen Y et al.Conversion of 5 methylcytosine to 5 hydroxymethylcytosine in mammalian DNA by MLL partner TET1[J].SCIENCE,2009,324(5929):930-935. |
| [8] | Shinsuke Ito;Li Shen;Qing Dai;Susan C. Wu;Leonard B. Collins;James A. Swenberg;Chuan He;Yi Zhang .Tet Proteins Can Convert 5-Methylcytosine to 5-Formylcytosine and 5-Carboxylcytosine[J].Science,2011(Sep.2 TN.6047):1300-1303. |
| [9] | Yu-Fei He;Bin-Zhong Li;Zheng Li;Peng Liu;Yang Wang;Qingyu Tang;Jianping Ding;Yingying Jia;Zhangcheng Chen;Lin Li;Yan Sun;Xiuxue Li;Qing Dai;Chun-Xiao Song;Kangling Zhang;Chuan He;Guo-Liang Xu .Tet-Mediated Formation of 5-Carboxylcytosine and Its Excision by TDG in Mammalian DNA[J].Science,2011(Sep.2 TN.6047):1303-1307. |
| [10] | Toni Pfaffeneder;Benjamin Hackner;Matthias Truss .The Discovery of 5-Formylcytosine in Embryonic Stem Cell DNA[J].Angewandte Chemie,2011(31):7008-7012. |
| [11] | TIAN Tian,WANG ShaoRu,WU JianGuo,ZHOU Xiang.Review: Advances in methodology of DNA methylation assay[J].中国科学:化学（英文版）,2011(08):1233-1243. |
| [12] | R Shapiro;R E Servis;M Welcher .Reactions of uracil and cytosine derivatives with sodium bisulfite[J].Journal of the American Chemical Society,1970,92:422-424. |
| [13] | H Hayatsu;Y Wataya;K Kai .Addition of sodium bisulfite to uracil and to cytosine[J].Journal of the American Chemical Society,1970,92:724-726. |
| [14] | Human DNA methylomes at base resolution show widespread epigenomic differences[J].Nature,2009(Nov.19 TN.7271):315. |
| [15] | Shankar Balasubramanian .Decoding Genomes at High Speed: Implications for Science and Medicine[J].Angewandte Chemie,2011(52):12406-12410. |
| [16] | Peter Schuler;Aubry K. Miller .Sequencing the Sixth Base (5-Hydroxymethylcytosine): Selective DNA Oxidation Enables Base-Pair Resolution[J].Angewandte Chemie,2012(43):10704-10707. |
| [17] | Wang, T.;Hong, T.;Tang, T.;Zhai, Q.;Xing, X.;Mao, W.;Zheng, X.;Xu, L.;Wu, J.;Weng, X.;Wang, S.;Tian, T.;Yuan, B.;Huang, B.;Zhuang, L.;Zhou, X. .Application of N-halogeno-N-sodiobenzenesulfonamide reagents to the selective detection of 5-methylcytosine in DNA sequences[J].Journal of the American Chemical Society,2013(4):1240-1243. |
| [18] | Longo M C;Berninger M S;Hartley J L .Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions[J].GENE,1990,93(01):125-128. |
| [19] | Huang, R.;Wang, J.;Mao, W.;Fu, B.;Xing, X.;Guo, G.;Zhou, X..A novel combined bisulfite UDG assay for selective 5-methylcytosine detection[J].Talanta: The International Journal of Pure and Applied Analytical Chemistry,2013:445-448. |
| [20] | Jin SG;Jiang Y;Qiu R;Rauch TA;Wang Y;Schackert G;Krex D;Lu Q;Pfeifer GP .5-Hydroxymethylcytosine is strongly depleted in human cancers but its levels do not correlate with IDH1 mutations.[J].Cancer research: The official organ of the American Association for Cancer Research, Inc,2011(24):7360-7365. |
| [21] | Lian, C.G.;Xu, Y.;Ceol, C.;Wu, F.;Larson, A.;Dresser, K.;Xu, W.;Tan, L.;Hu, Y.;Zhan, Q.;Lee, C.-W.;Hu, D.;Lian, B.Q.;Kleffel, S.;Yang, Y.;Neiswender, J.;Khorasani, A.J.;Fang, R.;Lezcano, C.;Duncan, L.M.;Scolyer, R.A.;Thompson, J.F.;Kakavand, H.;Houvras, Y.;Zon, L.I. .Loss of 5-hydroxymethylcytosine is an epigenetic hallmark of Melanoma[J].Cell,2012(6):1135-1146. |
| [22] | Huang Y;Pastor W A;Shen Y et al.The behaviour of 5-hydroxymethylcytosine in bisulfite sequencing[J].PLoS One,2010,5(01):e8888. |
| [23] | Jin S G;Kadam S;Pfeifer G P .Examination of the specificity of DNA methylation profiling techniques towards 5-methylcytosine and 5-hydroxymethylcytosine[J].Nucleic Acids Research,2010,38(11):e125-e125. |
| [24] | Hayatsu H;Shiragami M .Reaction of bisulfite with the 5-hydroxymethyl group in pyrimidines and in phage DNAs[J].Biochemistry,1979,18(04):632-637. |
| [25] | Yu, M.;Hon, G.C.;Szulwach, K.E.;Song, C.-X.;Zhang, L.;Kim, A.;Li, X.;Dai, Q.;Shen, Y.;Park, B.;Min, J.-H.;Jin, P.;Ren, B.;He, C. .Base-resolution analysis of 5-hydroxymethylcytosine in the mammalian genome[J].Cell,2012(6):1368-1380. |
| [26] | Michael J. Booth;Miguel R. Branco;Gabriella Ficz;David Oxley;Felix Krueger;Wolf Reik;Shankar Balasubramanian .Quantitative Sequencing of 5-Methylcytosine and 5-Hydroxymethylcytosine at Single-Base Resolution[J].Science,2012(TN.6083):934-937. |
| [27] | Song C X;Szulwach K E;Fu Y et al.Selective chemical labeling reveals the genome-wide distribution of 5-hydroxy methylcytosine[J].Nature Biotechnology,2010,29(01):68-72. |
| [28] | Song, C.-X.;Clark, T.A.;Lu, X.-Y.;Kislyuk, A.;Dai, Q.;Turner, S.W.;He, C.;Korlach, J. .Sensitive and specific single-molecule sequencing of 5- hydroxymethylcytosine[J].Nature methods,2012(1):75-77. |
| [29] | William A. Pastor;Utz J. Pape;Yun Huang;Hope R. Henderson;Ryan Lister;Myunggon Ko;Erin M. McLoughlin;Yevgeny Brudno;Sahasransu Mahapatra;Philipp Kapranov;Mamta Tahiliani;George Q. Daley;X. Shirley Liu;Joseph R. Ecker;Patrice M. Milos;Suneet Agarwal;Anjana Rao .Genome-wide mapping of 5-hydroxymethylcytosine in embryonic stem cells[J].Nature,2011(TN.7347):394-397. |
| [30] | Okamoto, A.;Sugizaki, K.;Nakamura, A.;Yanagisawa, H.;Ikeda, S. .5-Hydroxymethylcytosine-selective oxidation with peroxotungstate[J].Chemical communications,2011(40):11231-11233. |
| [31] | Zhou X;Yan S;Xu X et al.A turn-on fluorescence strategy by selective one-step reaction with 5-hydroxymethyl-2'-deoxycytidine for the formation of 1,3-O,N-heterocycles[J].RSCAdvances,2013,3:12066-12068. |
| [32] | Hong T T;Wang T;Guo P et al.Fluorescent strategy based on CCP-FRET for the quantification of 5-hydroxymethylcytosine in genomic DNA[J].Analytical Chemistry,2013,85:10797-10802. |
| [33] | Cadet J;Berger M .Radiation-induced decomposition of the purine bases within DNA and related model compounds[J].International Journal of Radiation Oncology,1985,47(02):127-143. |
| [34] | Djuric Z;Heilbrun L K;Simon M S et al.Levels of 5-hydroxymethyl-2'-deoxyuridine in DNA from blood as a marker of breast cancer[J].CANCER,1996,77(04):691-696. |
| [35] | Bhutani, N.;Burns, D.M.;Blau, H.M. .DNA demethylation dynamics[J].Cell,2011(6):866-872. |
| [36] | Hong IS;Ding H;Greenberg MM .Oxygen independent DNA interstrand cross-link formation by a nucleotide radical[J].Journal of the American Chemical Society,2006(2):485-491. |
| [37] | Xu, X.;Yan, S.;Hu, J.;Guo, P.;Wei, L.;Weng, X.;Zhou, X. .One-step to get 5-azidomethyl-2'-deoxyuridine from 5-hydroxymethyl-2'-deoxyuridine and detection of it through click reaction[J].Tetrahedron,2013(46):9870-9874. |
| [38] | Akimitsu Okamoto;Kazuki Tainaka;Isao Saito .A Dielectric-Sensitive Fluorescent DNA Probe for Monitoring Polarities on the Interior of a DNA-Binding Protein[J].Bioconjugate Chemistry,2005(5):1105-1111. |
| [39] | Cohen BE;McAnaney TB;Park ES;Jan YN;Boxer SG;Jan LY .Probing protein electrostatics with a synthetic fluorescent amino acid[J].Science,2002(5573):1700-1703. |
| [40] | Okamoto, A .Chemical approach toward efficient DNA methylation analysis[J].Organic & biomolecular chemistry,2009(1):21-26. |
| [41] | Münzel, M.;Lischke, U.;Stathis, D.;Pfaffeneder, T.;Gnerlich, F.A.;Deiml, C.A.;Koch, S.C.;Karaghiosoff, K.;Carell, T. .Improved synthesis and mutagenicity of oligonucleotides containing 5-hydroxymethylcytosine, 5-formylcytosine and 5-carboxylcytosine[J].Chemistry: A European journal,2011(49):13782-13788. |
| [42] | Raiber E A;Beraldi D;Ficz G et al.Genome-wide distribution of 5-formylcytosine in embryonic stem cells is associated with transcription and depends on thymine DNA glyco sylase[J].GENOME BIOLOGY,2012,13(08):R69. |
| [43] | Hu, J.;Xing, X.;Xu, X.;Wu, F.;Guo, P.;Yan, S.;Xu, Z.;Xu, J.;Weng, X.;Zhou, X. .Selective chemical labelling of 5-formylcytosine in DNA by fluorescent dyes[J].Chemistry: A European journal,2013(19):5836-5840. |
| [44] | Guo, P.;Yan, S.;Hu, J.;Xing, X.;Wang, C.;Xu, X.;Qiu, X.;Ma, W.;Lu, C.;Weng, X.;Zhou, X. .Selective detection of 5-formyl-2′-deoxycytidine in DNA using a fluorogenic hydroxylamine reagent[J].Organic letters,2013(13):3266-3269. |
| [45] | Mao, W.;Hu, J.;Hong, T.;Xing, X.;Wang, S.;Chen, X.;Zhou, X. .A convenient method for selective detection of 5-hydroxymethylcytosine and 5-formylcytosine sites in DNA sequences[J].Organic & biomolecular chemistry,2013(21):3568-3572. |
| [46] | Tian, T.;Zhang, X.;Fu, B.;Long, Y.;Peng, S.;Wang, S.;Zhou, X. .Systematic investigation of DNAs with modified cytosines under hot alkali treatment[J].Chemical communications,2013(85):9968-9970. |
| [47] | Xing X W;Liu Y L;Vargas M et al.Mutagenic and cytotoxic properties of oxidation products of 5-methylcytosine revealed by next-generation sequencing[J].PloS One,2013,8(09):e72993. |
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