为揭示生态强化法原位净化村镇污水机制与微生物特性，以常州市武进区前黄镇前桥污水治理示范工程底泥中微生物为研究考察对象，通过倒置显微镜镜检、磷脂脂肪酸（ PLFA）分析手段对其进行了系统研究．镜检显示底泥中含有原生动物、轮虫以及菌藻． PLFA 分析表明，底泥中饱和脂肪酸含量最为丰富，占76?97％；其次为单不饱和脂肪酸，占19．07％；最少是多不饱和脂肪酸，占3．96％．以脂肪酸生物标记量为指标，表明底泥的生态群落中是以假单胞杆菌、好氧细菌为主导．生态强化法原位净化村镇污水可能主要通过微生物的分解、水生植物的吸收以及藻类?水生动植物?细菌的协同作用实现对污染物的去除．
To investigate the microbial characteristic of in?situ strengthening bioremediation of rural domestic sewage, sediment microorganisms of Qianqiao demonstration project for rural domestic sewage treatment in Wujin,changzhou as the object of investigation were examined by phospholipid fatty acid method(PLFA).Microscopic examination showed that there were protozoa,rotifera,bacteria and algae in the sediment. PLFA analysis showed that total PLFAs were composed as follows, 76?97% saturated fatty acid,19.07% monounsaturated fatty acid,3.96% polyunsaturated fatty acid. Index of PLFA biomarkers volume showed pseudomonas and aerobic bacteria were predominant in the sediment microorganisms. It was proposed showed that the pollutants were mostly removed by microbial decomposition and plant uptaking, as well as synergistic effect of algae?aquatic animals/plants?bacteria.
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