采用清远电子垃圾拆解区附近河流底泥作为菌源，研究了四溴双酚A（ TBBPA）在不同厌氧条件下的还原脱溴，并用驯化微生物好氧降解双酚A（ BPA）。实验发现，添加电子供体和产甲烷条件下，TBBPA分别在117 d和42 d实现100％去除。硫酸盐条件下，TBBPA在观察期内（160 d）的去除率为63％。驯化微生物可以将BPA矿化，并在pH 8、34℃、接种量10％条件下，40 mg·L－1 BPA在6 d内可被完全降解，具有最优的降解效果。 BPA经对羟基苯乙酮、乳酸而最终转化为CO2和水。厌氧?好氧微生物作用可实现TBBPA的彻底降解，为受TBBPA污染区域的原位修复提供了科学基础。
Tetrabromobisphenol A ( TBBPA) is the most widely used brominated flame retardant and a potential endocrine disruptor. In sediments, TBBPA is mainly removed by anaerobic debromination while its ultimate debromination product, bisphenol A ( BPA) , still possesses estrogenic activity. In this study, the effects of different anaerobic conditions on transformation efficiency of TBBPA in river sediments near an electronic waste processing facility and aerobic degradation of BPA by domesticated microorganism were investigated. The results showed that TBBPA was completely removed within 117 d and 42 d under electron donor and methanogenic conditions respectively, and the removal ratio was 63% at 160 d under sulfate reducing condition. The optimal conditions for the degradation of 40 mg·L-1 BPA were at 34 ℃, initial pH 8 and 10% inoculum volume. BPA was metabolized to 4?hydroxyacetophenone and 2?hydroxypropanoic acid, and mineralized in the end. Complete degradation of TBBPA by anaerobic and aerobic microbial action provides scientific foundation to in situ remediation of contamination by TBBPA.
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