本文利用MoS2纳米片优异的荧光淬灭能力以及其与DNA分子之间的相互作用,构建了一种检测Hg2+的新型荧光生物传感器,并考察了其用于饮用水中微量Hg2+测定的可行性.实验优化了MoS2纳米片浓度、pH值、盐浓度及反应时间等参数对荧光生物传感器性能的影响.在此基础上建立了测定饮用水中微量Hg2+的荧光新方法.在最佳条件下,Hg2+浓度在10-900 nmol· L-1范围内与荧光强度的相对值具有良好的线性关系,检测限为6.3 nmol· L-1.该方法简单、灵敏、特异性强.该方法已成功用于饮用水中微量Hg2+的测定,回收率为95.7％-103.3％.该i究工作将MoS2纳米片的应用拓展到了环境监测领域,这对无机类石墨烯材料的深入研究具有重要意义.
In this work,a novel fluorescence biosensor based on MoS2 nanosheets and functional nucleic acids has been developed to detect Hg2+ in drinking water.This biosensor was constituted by exploiting high fluorescence quenching ability of MoS2 nanosheets and the interactions between MoS2 nanosheets and DNA molecules.Several analytical parameters including the concentration of MoS2 nanosheets,pH value,the concentration of salts,and the reaction time were investigated and optimized.Under the optimal conditions,a linear relationship between the concentration of Hg2+ and the fluorescence intensity was obtained over the range of 10 to 900 nmol· L-1 with a detection limit of 6.3 nmol· L-1.The recovery in water samples was in the range of 95.7％-103.3％.As the main advantages of this method were simple,sensitive,and specific,it holds great potential for Hg2+ detection in drinking water.This work also extended the application of MoS2 nanosheets to environmental monitoring.
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