本文选取了理化性质差异较大的3个区域的土壤作为研究对象,采用底物诱导呼吸实验进行土壤微生物活性测定,并通过磷脂脂肪酸(PLFA)方法进行微生物群落结构检测分析.结果表明,AM真菌可作为土壤微生物活性的指示性菌种;主成分分析表明,重金属对不同区域土壤的微生物群落结构、分布和疏密度的影响有一定差异性,即对于受污染胁迫后稳定性较好的土壤,其微生物群落结构基本会保持整体上的相似和不变性,而对稳定性较差的土壤,受重金属污染前后其微生物多样性、均匀度和丰度均会表现出明显的差异性;聚类分析则显示,土壤微生物群落的聚类会随时间增加趋于统一,但重金属胁迫会阻碍这一趋势.本研究探讨了重金属污染与土壤微生物的内在关系,有助于提早构建土壤重金属污染微生物预警体系.
In this study,soil samples were selected from 3 regions in China with obvious variation of physico-chemical properties.The soil incubation with a measurement of respiration was performed to observe the soil microbial activities,and the method of phospholipid fatty acids (PLFA) was applied for analyzing the microbial community structure.The results show that AM fungi could be used as indicative bacteria of soil microbial activity;and the principal component analysis shows that heavy metal stress would have certain influence on microbial PLFA's species,distribution and density.For the soil with high stability,its microbial community structure remained the overall similarities and invariance;while for the soil with poor stability,the microbial diversity and evenness showed obvious differences.Clustering analysis shows that the diversity of soil microbial communities over time tends to be uniform,and heavy metals stress will hinder this trend.This study discussed the relationship between heavy metal pollution and soil microorganism,which can be contributed to the early warning system of heavy metal pollution for soil ecosystem.
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