利用毛细管电泳(Capillary electrophoresis,CE)建立邻苯二甲酸二甲酯(Dimethyl Phthalate,DMP)-牛血清白蛋白(Bovine Serum Albumin,BSA)相互作用的分析方法.构建配体DMP-受体BSA相互作用模型.通过区段-区段动力学法(Plug-plug kinetic,PPK),HD法(Hummel-Dreyer,HD),前沿分析法(Frontal analysis,FA)和空峰法(Vacant peak,VP)测定DMP与BSA的相互作用,分别运用Scatchard方程,Klotz方程和非线性方程(Non-linear regression)等理论方法获取两者的相互作用参数,进而分析理论模型适用度.结果表明,PPK法、HD法与FA法都适用于DMP-BSA体系相互作用的测定,HD法最优.模型适用度分析得出非线性回归方程为最适合的理论模型.相互作用参数测试表明DMP-BSA只有单一类型的结合位点且结合强度适中,DMP-BSA分子水平的测定结果可为DMP毒理机制的深入研究提供有益参考.
The interaction between dimethyl phthalate (DMP) and bovine serum albumin (BSA) was investigated by capillary electrophoresis (CE).The binding models of DMP (as the ligand) to BSA(as the receptor) were developped.Plug-plug kinetic method (PPK),Hummel-Dreyer method (HD),Frontal analysis method (FA) and Vacant peak method (VP) were used to explore the interaction parameters of the DMP-BSA system.Scatchard,Klotz and non-linear regression equations were adopted to obtain interaction parameters of the system,and the feasible modeling equation was hypothesized.The results showed that PPK,HD and FA can all be used to analyze the DMP-BSA system,and the most suitable CE method is HD.According to the obtained interaction parameters,the most suitable equation is the regression method equation for this system through careful comparative analysis.The binding parameters indicated that the interaction between DMP-BSA was moderate,which had only one type of binding sites.
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