本研究利用微流体芯片配合雷射激发荧光侦测法,侦测食品中不当添加物一甲醛,实验中以4-amino-3-penten-2-one(Fluoral-P)和甲醛作用,并于微流体混合器中进行反应及荧光衍生化以快速侦测.在微流体芯片中过高浓度甲醛荧光衍生物,因荧光分子基团讯号集中,易干扰全波长与荧光的侦测,因此在微流体芯片的检测以100 ppm以下为主.而在微流体混合器方面,本研究利用1:7型式不对称管道之微流体混合器,甲醛于此芯片中行荧光侦测法测定,随着甲醛浓度的降低,荧光讯号愈平缓,易出现噪声,侦测极限可达0.4 ppm且其线性度为R2=0.9954,而浓度低于1 ppm时,其S/N比表现度随之降低,此系统可提供较短分析时间、低试剂与成本消耗.
参考文献
| [1] | Hui-Ling Lee;Show-Chuen Chen .Microchip capillary electrophoresis with amperometric detection for several carbohydrates[J].Talanta: The International Journal of Pure and Applied Analytical Chemistry,2004(1):210-216. |
| [2] | Dolnik V;Liu S .Application of capillary electrophoresis on microchip[J].Journal of Sep Science,2005,28(15):1994-2009. |
| [3] | Kato M;Gyoten Y;Sakai-Kato K et al.Rapid analysis of amino acids in Japanese green tea by microchip eleetroph[J].oresis using plastic microchip and fluorescence detection J Chromatogr A,2003,1013(1-2):183-189. |
| [4] | Scampicchio M;Wang J;Mannino S;Chatrathi MP .Microchip capillary electrophoresis with amperometric detection for rapid separation and detection of phenolic acids[J].Journal of chromatography, A: Including electrophoresis and other separation methods,2004(1/2):189-194. |
| [5] | Law WS;Kuban P;Zhao JH;Li SF;Hauser PC .Determination of vitamin C and preservatives in beverages by conventional capillary electrophoresis and microchip electrophoresis with capacitively coupled contactless conductivity detection.[J].Electrophoresis: The Official Journal of the International Electrophoresis Society,2005(24):4648-4655. |
| [6] | Hattan DG;Kahl LS .Current developments in food additive toxicology in the USA.[J].Toxicology: An International Journal Concerned with the Effects of Chemicals on Living Systems,2002(0):417-420. |
| [7] | Hamano T;Mitsuhashi Y;Yamamoto S .Determination of hydrogen peroxide in beverages by high performance liquid chromatography with fluorescence detection[J].Journal of Chromatography,1978,411(05):423-429. |
| [8] | Che-Hsin Lin;Chien-Hsiung Tsai;Chih-Wen Pan;Lung-Ming Fu .Rapid Circular Microfluidic Mixer Utilizing Unbalanced Driving Force[J].Biomedical microdevices,2007(1):43-50. |
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